biostudies-literatureUnknown9(11)Gomez-Orte EThe transcriptomes of model organisms have been defined under specific laboratory growth conditions. The standard protocol for <i>Caenorhabditis elegans</i> growth and maintenance is 20°C on an <i>Escherichia coli</i> diet. Temperatures ranging from 15°C to 25°C or feeding with other species of bacteria are considered physiological conditions, but the effect of these conditions on the worm transcriptome has not been well characterized. Here, we compare the global gene expression profile for the reference <i>Caenorhabditis elegans</i> strain (N2) grown at 15°C, 20°C, and 25°C on two different diets, <i>Escherichia coli</i> and <i>Bacillus subtilis</i>. When <i>C. elegans</i> were fed <i>E. coli</i> and the growth temperature was increased, we observed an enhancement of defense response pathOncotarget9556-9571https://www.ebi.ac.uk/biostudies/studies/S-EPMC5839384biostudies-literatureEffect of the diet type and temperature on the <i>C. elegans</i> transcriptome.PMC5839384Saenz-Narciso BIniguez MCabello JSan-Juan JFde Toro MLopez RGomez-Orte EZheleva ACeron JSacristan BEzcurra BCornes ESanchez-Blanco AfalseEffect of the diet type and temperature on the <i>C. elegans</i> transcriptome.The transcriptomes of model organisms have been defined under specific laboratory growth conditions. The standard protocol for <i>Caenorhabditis elegans</i> growth and maintenance is 20°C on an <i>Escherichia coli</i> diet. Temperatures ranging from 15°C to 25°C or feeding with other species of bacteria are considered physiological conditions, but the effect of these conditions on the worm transcriptome has not been well characterized. Here, we compare the global gene expression profile for the reference <i>Caenorhabditis elegans</i> strain (N2) grown at 15°C, 20°C, and 25°C on two different diets, <i>Escherichia coli</i> and <i>Bacillus subtilis</i>. When <i>C. elegans</i> were fed <i>E. coli</i> and the growth temperature was increased, we observed an enhancement of defense response path2018-01-01T00:00:00Z2018 Feb2026-04-30T09:45:08.211Z2019-03-26T23:06:56ZS-EPMC58393842951575310.18632/oncotarget.23563