biostudies-literatureDing CJiangsu Provincial medical innovation teamSuzhou key medicine centerSuzhou Health Bureau fundProject funded by China Postdoctoral Science FundationSuzhou science and technology for people's livelihoodSuzhou introduce expert team of clinical medicineMaternal and health care project of Jiangsu Province- assisted reproduction medicine center55https://www.ebi.ac.uk/biostudies/studies/S-EPMC5845161biostudies-literatureUnknown9(1)BACKGROUND:Although many reports show that various kinds of stem cells have the ability to recover function in premature ovarian aging, few studies have looked at stem cell treatment of natural ovarian aging (NOA). We designed this experimental study to investigate whether human amniotic mesenchymal stem cells (hAMSCs) retain the ability to restore ovarian function, and how hAMSCs work in this process. METHODS:To build the NOA mouse model, the mice were fed for 12-14 months normally with young fertile female mice as the normal control group (3-5 months old). Hematoxylin and eosin staining permitted follicle counting and showed the ovarian tissue structure. An enzyme-linked immunosorbent assay was used to detect the serum levels of the sex hormones estradiol (E2), anti-mullerian hormone (AMStem cell research & therapyHuman amniotic mesenchymal stem cells improve ovarian function in natural aging through secreting hepatocyte growth factor and epidermal growth factor.PMC5845161SYS201771FKX201224SZZX201505SZYJTD2017082017M611871CXTDB2017013lczx201411Lv JLi HDing CLing MZou QWang FWang WChen RHuang BSun JWu HfalseHuman amniotic mesenchymal stem cells improve ovarian function in natural aging through secreting hepatocyte growth factor and epidermal growth factor.BACKGROUND:Although many reports show that various kinds of stem cells have the ability to recover function in premature ovarian aging, few studies have looked at stem cell treatment of natural ovarian aging (NOA). We designed this experimental study to investigate whether human amniotic mesenchymal stem cells (hAMSCs) retain the ability to restore ovarian function, and how hAMSCs work in this process. METHODS:To build the NOA mouse model, the mice were fed for 12-14 months normally with young fertile female mice as the normal control group (3-5 months old). Hematoxylin and eosin staining permitted follicle counting and showed the ovarian tissue structure. An enzyme-linked immunosorbent assay was used to detect the serum levels of the sex hormones estradiol (E2), anti-mullerian hormone (AM2018-01-01T00:00:00Z2018 Mar2025-04-04T09:05:36.138Z2019-03-26T23:17:59ZS-EPMC58451612952319310.1186/s13287-018-0781-9