{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["13"],"submitter":["Muzaffer U"],"pubmed_abstract":["The present study was aimed to investigate the photoprotective effect of the male flower of J. regia L. (MEJR) against ultraviolet-B induced apoptosis in human skin cells. Human skin epidermal keratinocytes were pretreated with the MEJR (80 µg/ml, has been selected after MTT assay), prior to 30 min UVB-irradiation at a dose of 20 mJ/cm2. Mitochondrial membrane potential was evaluated using Rhodamine-123 staining; the % apoptosis by Hoechst staining and acridine orange staining; DNA damage was measured by comet assay. The levels of p53, Bax, Bcl-xL, Bcl-2, Cytochrome c, Caspase-9 and Caspase-3 expression in HaCaT cells were analyzed by western blotting and RT-PCR. Pretreatment with MEJR 80 µg/ml prior to UVB-irradiation significantly prevents apoptotic characteristics, DNA damage and loss o"],"journal":["Biochemistry and biophysics reports"],"pagination":["109-115"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC5857159"],"repository":["biostudies-literature"],"pubmed_title":["Juglans regia L. protects against UVB induced apoptosis in human epidermal keratinocytes."],"pmcid":["PMC5857159"],"pubmed_authors":["Paul VI","Karthikeyan R","Muzaffer U","Prasad NR"],"additional_accession":[]},"is_claimable":false,"name":"Juglans regia L. protects against UVB induced apoptosis in human epidermal keratinocytes.","description":"The present study was aimed to investigate the photoprotective effect of the male flower of J. regia L. (MEJR) against ultraviolet-B induced apoptosis in human skin cells. Human skin epidermal keratinocytes were pretreated with the MEJR (80 µg/ml, has been selected after MTT assay), prior to 30 min UVB-irradiation at a dose of 20 mJ/cm2. Mitochondrial membrane potential was evaluated using Rhodamine-123 staining; the % apoptosis by Hoechst staining and acridine orange staining; DNA damage was measured by comet assay. The levels of p53, Bax, Bcl-xL, Bcl-2, Cytochrome c, Caspase-9 and Caspase-3 expression in HaCaT cells were analyzed by western blotting and RT-PCR. Pretreatment with MEJR 80 µg/ml prior to UVB-irradiation significantly prevents apoptotic characteristics, DNA damage and loss o","dates":{"release":"2018-01-01T00:00:00Z","publication":"2018 Mar","modification":"2025-04-19T13:15:30.058Z","creation":"2019-03-26T23:20:15Z"},"accession":"S-EPMC5857159","cross_references":{"pubmed":["29556565"],"doi":["10.1016/j.bbrep.2018.01.004"]}}