biostudies-literatureGokey TNational Institute of Allergy and Infectious DiseasesSan Francisco State UniversityU.S. Department of Health and Human ServicesNIAID NIH HHSNational Institutes of Health175-181https://www.ebi.ac.uk/biostudies/studies/S-EPMC5864537biostudies-literatureUnknown202(2)Many bacteria require l-rhamnose as a key cell wall component. This sugar is transferred to the cell wall using an activated donor dTDP-l-rhamnose, which is produced by the dTDP-l-rhamnose biosynthetic pathway. We determined the crystal structure of the second enzyme of this pathway dTDP-α-d-glucose 4,6-dehydratase (RfbB) from Bacillus anthracis. Interestingly, RfbB only crystallized in the presence of the third enzyme of the pathway RfbC; however, RfbC was not present in the crystal. Our work represents the first complete structural characterization of the four proteins of this pathway in a single Gram-positive bacterium.Journal of structural biologyStructure of the Bacillus anthracis dTDP-l-rhamnose biosynthetic pathway enzyme: dTDP-α-d-glucose 4,6-dehydratase, RfbB.PMC5864537HHSN272201200026CHHSN272200700058CHalavaty ASAnderson WFKuhn MLGokey TMinasov GfalseStructure of the Bacillus anthracis dTDP-l-rhamnose biosynthetic pathway enzyme: dTDP-α-d-glucose 4,6-dehydratase, RfbB.Many bacteria require l-rhamnose as a key cell wall component. This sugar is transferred to the cell wall using an activated donor dTDP-l-rhamnose, which is produced by the dTDP-l-rhamnose biosynthetic pathway. We determined the crystal structure of the second enzyme of this pathway dTDP-α-d-glucose 4,6-dehydratase (RfbB) from Bacillus anthracis. Interestingly, RfbB only crystallized in the presence of the third enzyme of the pathway RfbC; however, RfbC was not present in the crystal. Our work represents the first complete structural characterization of the four proteins of this pathway in a single Gram-positive bacterium.2018-01-01T00:00:00Z2018 May2024-11-19T19:00:00.885Z2019-06-06T22:47:56ZS-EPMC58645372933160910.1016/j.jsb.2018.01.006