{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Zhang Y"],"funding":["American Heart Association"],"pagination":["e00743-17"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC5869477"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["200(8)"],"pubmed_abstract":["Clustered regularly interspaced short palindromic repeats (CRISPR) and the CRISPR-associated proteins (Cas) provide bacteria and archaea with adaptive immunity to specific DNA invaders. <i>Mycobacterium tuberculosis</i> encodes a type III CRISPR-Cas system that has not been experimentally explored. In this study, we found that the CRISPR-Cas systems of both <i>M. tuberculosis</i> and <i>Mycobacterium bovis</i> BCG were highly upregulated by deletion of Rv2837c (<i>cnpB</i>), which encodes a multifunctional protein that hydrolyzes cyclic di-AMP (c-di-AMP), cyclic di-GMP (c-di-GMP), and nanoRNAs (short oligonucleotides of 5 or fewer residues). By using genetic and biochemical approaches, we demonstrated that the CnpB-controlled transcriptional regulation of the CRISPR-Cas system is mediated "],"journal":["Journal of bacteriology"],"pubmed_title":["Regulation of the CRISPR-Associated Genes by Rv2837c (CnpB) via an Orn-Like Activity in Tuberculosis Complex Mycobacteria."],"pmcid":["PMC5869477"],"funding_grant_id":["12SDG12080067"],"pubmed_authors":["Yang J","Zhang Y","Bai G"],"additional_accession":[]},"is_claimable":false,"name":"Regulation of the CRISPR-Associated Genes by Rv2837c (CnpB) via an Orn-Like Activity in Tuberculosis Complex Mycobacteria.","description":"Clustered regularly interspaced short palindromic repeats (CRISPR) and the CRISPR-associated proteins (Cas) provide bacteria and archaea with adaptive immunity to specific DNA invaders. <i>Mycobacterium tuberculosis</i> encodes a type III CRISPR-Cas system that has not been experimentally explored. In this study, we found that the CRISPR-Cas systems of both <i>M. tuberculosis</i> and <i>Mycobacterium bovis</i> BCG were highly upregulated by deletion of Rv2837c (<i>cnpB</i>), which encodes a multifunctional protein that hydrolyzes cyclic di-AMP (c-di-AMP), cyclic di-GMP (c-di-GMP), and nanoRNAs (short oligonucleotides of 5 or fewer residues). By using genetic and biochemical approaches, we demonstrated that the CnpB-controlled transcriptional regulation of the CRISPR-Cas system is mediated ","dates":{"release":"2018-01-01T00:00:00Z","publication":"2018 Apr","modification":"2026-04-29T16:59:13.975Z","creation":"2019-03-26T23:57:04Z"},"accession":"S-EPMC5869477","cross_references":{"pubmed":["29378893"],"doi":["10.1128/JB.00743-17","10.1128/jb.00743-17"]}}