biostudies-literatureZhang YAmerican Heart Associatione00743-17https://www.ebi.ac.uk/biostudies/studies/S-EPMC5869477biostudies-literatureUnknown200(8)Clustered regularly interspaced short palindromic repeats (CRISPR) and the CRISPR-associated proteins (Cas) provide bacteria and archaea with adaptive immunity to specific DNA invaders. <i>Mycobacterium tuberculosis</i> encodes a type III CRISPR-Cas system that has not been experimentally explored. In this study, we found that the CRISPR-Cas systems of both <i>M. tuberculosis</i> and <i>Mycobacterium bovis</i> BCG were highly upregulated by deletion of Rv2837c (<i>cnpB</i>), which encodes a multifunctional protein that hydrolyzes cyclic di-AMP (c-di-AMP), cyclic di-GMP (c-di-GMP), and nanoRNAs (short oligonucleotides of 5 or fewer residues). By using genetic and biochemical approaches, we demonstrated that the CnpB-controlled transcriptional regulation of the CRISPR-Cas system is mediated Journal of bacteriologyRegulation of the CRISPR-Associated Genes by Rv2837c (CnpB) via an Orn-Like Activity in Tuberculosis Complex Mycobacteria.PMC586947712SDG12080067Yang JZhang YBai GfalseRegulation of the CRISPR-Associated Genes by Rv2837c (CnpB) via an Orn-Like Activity in Tuberculosis Complex Mycobacteria.Clustered regularly interspaced short palindromic repeats (CRISPR) and the CRISPR-associated proteins (Cas) provide bacteria and archaea with adaptive immunity to specific DNA invaders. <i>Mycobacterium tuberculosis</i> encodes a type III CRISPR-Cas system that has not been experimentally explored. In this study, we found that the CRISPR-Cas systems of both <i>M. tuberculosis</i> and <i>Mycobacterium bovis</i> BCG were highly upregulated by deletion of Rv2837c (<i>cnpB</i>), which encodes a multifunctional protein that hydrolyzes cyclic di-AMP (c-di-AMP), cyclic di-GMP (c-di-GMP), and nanoRNAs (short oligonucleotides of 5 or fewer residues). By using genetic and biochemical approaches, we demonstrated that the CnpB-controlled transcriptional regulation of the CRISPR-Cas system is mediated 2018-01-01T00:00:00Z2018 Apr2026-04-29T16:59:13.975Z2019-03-26T23:57:04ZS-EPMC58694772937889310.1128/JB.00743-1710.1128/jb.00743-17