biostudies-literatureZhang DNIBIB NIH HHSMOSTNational Institute of HealthNational Natural Science Foundation of ChinaNIGMS NIH HHSNIH HHS024003https://www.ebi.ac.uk/biostudies/studies/S-EPMC5871576biostudies-literatureUnknown6(2)Fluorescent samples typically emit isotropically in all directions. Large lenses and other optical components are needed to capture a significant fraction of the emission, and complex confocal microscopes are required for high resolution focal-plane imaging. It is known that Bessel beams have remarkable properties of being able to travel over long distances, over 1000 times the wavelength, without diverging, and hence are called non-diffracting beams. In previous reports the Bessel beams were formed by an incident light source, typically with plane-wave illumination on a circular aperture. It was not known if Bessel beams could form from fluorescent light sources. We demonstrate transformation of the emission from fluorescent polystyrene spheres (FPS) into non-diverging beams which propagaMethods and applications in fluorescenceConversion of isotropic fluorescence into a long-range non-diverging beam.PMC58715762016YFA020060111374286R21 EB0189592013CBA0170361427818EB006521R21 GM107986GM107986EB018959 and OD019975S10 OD019975R01 EB006521Liu XZhang DWang PChen JWang RLakowicz JRZhu LZhan QKuang CRosenfeld MMing HBadugu RfalseConversion of isotropic fluorescence into a long-range non-diverging beam.Fluorescent samples typically emit isotropically in all directions. Large lenses and other optical components are needed to capture a significant fraction of the emission, and complex confocal microscopes are required for high resolution focal-plane imaging. It is known that Bessel beams have remarkable properties of being able to travel over long distances, over 1000 times the wavelength, without diverging, and hence are called non-diffracting beams. In previous reports the Bessel beams were formed by an incident light source, typically with plane-wave illumination on a circular aperture. It was not known if Bessel beams could form from fluorescent light sources. We demonstrate transformation of the emission from fluorescent polystyrene spheres (FPS) into non-diverging beams which propaga2018-01-01T00:00:00Z2018 Jan2025-04-26T06:51:32.7Z2019-03-26T23:20:55ZS-EPMC58715762911994610.1088/2050-6120/aa9949