{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Insley P"],"funding":["National Institute of Neurological Disorders and Stroke","NINDS NIH HHS","NIH HHS","National Institute of Child Health and Human Development"],"pagination":["e0194861"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC5874040"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["13(3)"],"pubmed_abstract":["The Caenorhabditis elegans cell lineage is nearly invariant. Whether this stereotyped cell-division pattern promotes reproducibility in cell shapes/positions is not generally known, as manual spatiotemporal cell-shape/position alignments are labor-intensive, and fully-automated methods are not described. Here, we report automated algorithms for spatiotemporal alignments of C. elegans embryos from pre-morphogenesis to motor-activity initiation. We use sparsely-labeled green-fluorescent nuclei and a pan-nuclear red-fluorescent reporter to register consecutive imaging time points and compare embryos. Using our method, we monitor early assembly of the nerve-ring (NR) brain neuropil. While NR pioneer neurons exhibit reproducible growth kinetics and axon positions, cell-body placements are variable. Thus, pioneer-neuron axon locations, but not cell-body positions, are under selection. We also show that anterior NR displacement in cam-1/ROR Wnt-receptor mutants is not an early NR assembly defect. Our results demonstrate the utility of automated spatiotemporal alignments of C. elegans embryos, and uncover key principles guiding nervous-system development in this animal."],"journal":["PloS one"],"pubmed_title":["Automated C. elegans embryo alignments reveal brain neuropil position invariance despite lax cell body placement."],"pmcid":["PMC5874040"],"funding_grant_id":["NS081490","R35 NS105094","R01NS064273","HD078703","R35NS105094","NS095795","R01NS095795","R01HD078703","NS064273","R01NS081490"],"pubmed_authors":["Insley P","Shaham S"],"additional_accession":[]},"is_claimable":false,"name":"Automated C. elegans embryo alignments reveal brain neuropil position invariance despite lax cell body placement.","description":"The Caenorhabditis elegans cell lineage is nearly invariant. Whether this stereotyped cell-division pattern promotes reproducibility in cell shapes/positions is not generally known, as manual spatiotemporal cell-shape/position alignments are labor-intensive, and fully-automated methods are not described. Here, we report automated algorithms for spatiotemporal alignments of C. elegans embryos from pre-morphogenesis to motor-activity initiation. We use sparsely-labeled green-fluorescent nuclei and a pan-nuclear red-fluorescent reporter to register consecutive imaging time points and compare embryos. Using our method, we monitor early assembly of the nerve-ring (NR) brain neuropil. While NR pioneer neurons exhibit reproducible growth kinetics and axon positions, cell-body placements are variable. Thus, pioneer-neuron axon locations, but not cell-body positions, are under selection. We also show that anterior NR displacement in cam-1/ROR Wnt-receptor mutants is not an early NR assembly defect. Our results demonstrate the utility of automated spatiotemporal alignments of C. elegans embryos, and uncover key principles guiding nervous-system development in this animal.","dates":{"release":"2018-01-01T00:00:00Z","publication":"2018","modification":"2024-12-04T00:32:55.163Z","creation":"2019-03-26T23:25:58Z"},"accession":"S-EPMC5874040","cross_references":{"pubmed":["29590193"],"doi":["10.1371/journal.pone.0194861"]}}