biostudies-literatureMor-Vaknin NNIDDK NIH HHSNIAID NIH HHSRheumatology Research FoundationNational Institutes of HealthCenter for Scientific ReviewNIAMS NIH HHS594-605https://www.ebi.ac.uk/biostudies/studies/S-EPMC5876119biostudies-literatureUnknown70(4)<h4>Objective</h4>The nuclear oncoprotein DEK is an autoantigen associated with juvenile idiopathic arthritis (JIA), especially the oligoarticular subtype. DEK is a secreted chemotactic factor. Abundant levels of DEK and DEK autoantibodies are found in inflamed synovium in JIA. We undertook this study to further characterize the nature of DEK autoantibodies in screening serum samples from 2 different cohorts that consisted mostly of patients with JIA.<h4>Methods</h4>DEK autoantibody levels were analyzed in sera from 33 JIA patients, 13 patients with other inflammatory conditions, and 11 healthy controls, as well as in 89 serum samples from JIA patients receiving anti-tumor necrosis factor (anti-TNF) therapy. Recombinant His-tagged full-length DEK protein (1-375 amino acids [aa]) and the 18Arthritis & rheumatology (Hoboken, N.J.)High Levels of DEK Autoantibodies in Sera of Patients With Polyarticular Juvenile Idiopathic Arthritis and With Early Disease Flares Following Cessation of Anti-Tumor Necrosis Factor Therapy.PMC5876119K01 AR055620T32 AI007413R01 AI062248R01 DK109188R01 AI087128R01‐AI‐087128R03‐AR‐056748‐01K01‐AR‐055620R01‐DK‐109188R03 AR056748R01‐AI‐062248P60 AR047784T32 AR007080Markovitz DMKimura YPasso MHCassidy EARivas MJung LKBeukelman TMehta JEdelheit BSZhao LGottlieb BSMohan SJohnson AAdams BSLovell DJSpalding SJHuang BOnel KMau TPrahalad SLegendre MGiannini EHOlson JCYuanfan YMor-Vaknin NMorris PWShishov MfalseHigh Levels of DEK Autoantibodies in Sera of Patients With Polyarticular Juvenile Idiopathic Arthritis and With Early Disease Flares Following Cessation of Anti-Tumor Necrosis Factor Therapy.<h4>Objective</h4>The nuclear oncoprotein DEK is an autoantigen associated with juvenile idiopathic arthritis (JIA), especially the oligoarticular subtype. DEK is a secreted chemotactic factor. Abundant levels of DEK and DEK autoantibodies are found in inflamed synovium in JIA. We undertook this study to further characterize the nature of DEK autoantibodies in screening serum samples from 2 different cohorts that consisted mostly of patients with JIA.<h4>Methods</h4>DEK autoantibody levels were analyzed in sera from 33 JIA patients, 13 patients with other inflammatory conditions, and 11 healthy controls, as well as in 89 serum samples from JIA patients receiving anti-tumor necrosis factor (anti-TNF) therapy. Recombinant His-tagged full-length DEK protein (1-375 amino acids [aa]) and the 182018-01-01T00:00:00Z2018 Apr2026-06-03T17:49:41.026Z2019-03-26T23:31:11ZS-EPMC58761192928730310.1002/art.40404