biostudies-literatureUnknown8(1)Su YThe biological pretreatment of lignocellulosic biomass is a low-cost and eco-friendly method for facilitating enzymatic hydrolysis. In this study, strains with lignin depletion capability were screened using a high-throughput screening method. Sixty-three strains were screened out and Myrothecium verrucaria secreted three lignin-degrading enzymes simultaneously during the bio-pretreatment process. The activity levels of laccase, lignin peroxidase and manganese peroxidase were 6.61, 0.78 and 1.31 U g-1 dry biomass. The content of lignin in corn stover decreased by 42.30% after bio-pretreatment, and the conversion rate increased by 123.84% during the subsequent saccharification process in comparison with the untreated corn stover. Furthermore, the effects of bio-pretreatment on the structureScientific reports5385https://www.ebi.ac.uk/biostudies/studies/S-EPMC5876370biostudies-literatureEvaluation of Screened Lignin-degrading Fungi for the Biological Pretreatment of Corn Stover.PMC5876370Chen GWang GChen HSu YSun YYu XfalseEvaluation of Screened Lignin-degrading Fungi for the Biological Pretreatment of Corn Stover.The biological pretreatment of lignocellulosic biomass is a low-cost and eco-friendly method for facilitating enzymatic hydrolysis. In this study, strains with lignin depletion capability were screened using a high-throughput screening method. Sixty-three strains were screened out and Myrothecium verrucaria secreted three lignin-degrading enzymes simultaneously during the bio-pretreatment process. The activity levels of laccase, lignin peroxidase and manganese peroxidase were 6.61, 0.78 and 1.31 U g-1 dry biomass. The content of lignin in corn stover decreased by 42.30% after bio-pretreatment, and the conversion rate increased by 123.84% during the subsequent saccharification process in comparison with the untreated corn stover. Furthermore, the effects of bio-pretreatment on the structure2018-01-01T00:00:00Z2018 Mar2025-04-04T09:05:37.095Z2019-03-26T23:23:25ZS-EPMC58763702959946510.1038/s41598-018-23626-6