biostudies-literatureLuengo-Gil GInstituto de Salud Carlos IIIFundación Salud 2000Ministerio de Educación, Cultura y Deportee0194638https://www.ebi.ac.uk/biostudies/studies/S-EPMC5884522biostudies-literatureUnknown13(4)<h4>Background</h4>Angiogenesis is a key process for tumor progression and a target for treatment. However, the regulation of breast cancer angiogenesis and its relevance for clinical resistance to antiangiogenic drugs is still incompletely understood. Recent developments on the contribution of microRNA to tumor angiogenesis and on the oncogenic effects of miR-17-92, a miRNA cluster, point to their potential role on breast cancer angiogenesis. The aim of this work was to establish the contribution of miR-20a, a member of miR-17-92 cluster, to tumor angiogenesis in patients with invasive breast carcinoma.<h4>Methods</h4>Tube-formation in vitro assays with conditioned medium from MCF7 and MDA-MB-231 breast cancer cell lines were performed after transfection with miR-20a and anti-miR20a. For PloS oneAngiogenic role of miR-20a in breast cancer.PMC5884522FPU16/06537Ayuda Merck Serono de Investigación en Oncología 2012PI12/02877Gonzalez-Billalabeitia ENavarro Manzano EGarcia-Garre EPerez-Henarejos SALuengo-Gil GGarcia-Martinez EChaves-Benito AAyala de la Pena FVicente VfalseAngiogenic role of miR-20a in breast cancer.<h4>Background</h4>Angiogenesis is a key process for tumor progression and a target for treatment. However, the regulation of breast cancer angiogenesis and its relevance for clinical resistance to antiangiogenic drugs is still incompletely understood. Recent developments on the contribution of microRNA to tumor angiogenesis and on the oncogenic effects of miR-17-92, a miRNA cluster, point to their potential role on breast cancer angiogenesis. The aim of this work was to establish the contribution of miR-20a, a member of miR-17-92 cluster, to tumor angiogenesis in patients with invasive breast carcinoma.<h4>Methods</h4>Tube-formation in vitro assays with conditioned medium from MCF7 and MDA-MB-231 breast cancer cell lines were performed after transfection with miR-20a and anti-miR20a. For 2018-01-01T00:00:00Z20182025-04-21T19:57:31.362Z2019-03-26T23:29:13ZS-EPMC58845222961740410.1371/journal.pone.0194638