{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Hiraike Y"],"funding":["NIDDK NIH HHS","NNF Center for Basic Metabolic Research"],"pagination":["1081-1092"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC5885759"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["19(9)"],"pubmed_abstract":["Brown fat dissipates energy as heat and protects against obesity. Here, we identified nuclear factor I-A (NFIA) as a transcriptional regulator of brown fat by a genome-wide open chromatin analysis of murine brown and white fat followed by motif analysis of brown-fat-specific open chromatin regions. NFIA and the master transcriptional regulator of adipogenesis, PPARγ, co-localize at the brown-fat-specific enhancers. Moreover, the binding of NFIA precedes and facilitates the binding of PPARγ, leading to increased chromatin accessibility and active transcription. Introduction of NFIA into myoblasts results in brown adipocyte differentiation. Conversely, the brown fat of NFIA-knockout mice displays impaired expression of the brown-fat-specific genes and reciprocal elevation of muscle genes. Fi"],"journal":["Nature cell biology"],"pubmed_title":["NFIA co-localizes with PPARγ and transcriptionally controls the brown fat gene program."],"pmcid":["PMC5885759"],"funding_grant_id":["R01 DK077097","Schéele Group","R01 DK102898"],"pubmed_authors":["Kadowaki T","Suzuki K","Kobayashi H","Hiraike Y","Yu J","Waki H","Nakaki R","Oki K","Scheele C","Tseng YH","Yoneda M","Hirota Y","Aoyama T","Tsutsumi S","Ohno H","Larsen TJ","Yamauchi T","Nakamura M","Miyake K","Aburatani H","Yamamoto S","White AP","Sun W","Jespersen NZ","Nagano G","Cypess AM"],"additional_accession":[]},"is_claimable":false,"name":"NFIA co-localizes with PPARγ and transcriptionally controls the brown fat gene program.","description":"Brown fat dissipates energy as heat and protects against obesity. Here, we identified nuclear factor I-A (NFIA) as a transcriptional regulator of brown fat by a genome-wide open chromatin analysis of murine brown and white fat followed by motif analysis of brown-fat-specific open chromatin regions. NFIA and the master transcriptional regulator of adipogenesis, PPARγ, co-localize at the brown-fat-specific enhancers. Moreover, the binding of NFIA precedes and facilitates the binding of PPARγ, leading to increased chromatin accessibility and active transcription. Introduction of NFIA into myoblasts results in brown adipocyte differentiation. Conversely, the brown fat of NFIA-knockout mice displays impaired expression of the brown-fat-specific genes and reciprocal elevation of muscle genes. Fi","dates":{"release":"2017-01-01T00:00:00Z","publication":"2017 Sep","modification":"2026-05-03T23:27:52.957Z","creation":"2019-03-26T23:26:04Z"},"accession":"S-EPMC5885759","cross_references":{"pubmed":["28812581"],"doi":["10.1038/ncb3590"]}}