biostudies-literatureUnknown18(1)Naidoo DBDepartment of Science and Technology, Government of IndiaManipal UniversityNational Research FoundationSouth African Medical Research Council<h4>Background</h4>Cancer and inflammation are associated with cachexia. Withania somnifera (W. somnifera) possesses antioxidant and anti-inflammatory potential. We investigated the potential of an aqueous extract of the root of W. somnifera (W_RE) to modulate cytokines, antioxidants and apoptosis in leukaemic THP-1 cells and peripheral blood mononuclear cells (PBMC's).<h4>Methods</h4>Cytotoxcity of W_RE was determined at 24 and 72 h (h). Oxidant scavenging activity of W_RE was evaluated (2, 2-diphenyl-1 picrylhydrazyl assay). Glutathione (GSH) levels, caspase (- 8, - 9, - 3/7) activities and adenosine triphosphate (ATP) levels (Luminometry) were thereafter assayed. Tumour necrosis factor-α (TNF-α), interleukin (IL)-6, IL-1β and IL-10 levels were also assessedBMC complementary and alternative medicine126https://www.ebi.ac.uk/biostudies/studies/S-EPMC5891897biostudies-literatureWithania somnifera modulates cancer cachexia associated inflammatory cytokines and cell death in leukaemic THP-1 cells and peripheral blood mononuclear cells (PBMC's).PMC5891897Chuturgoon AASatyamoorthy KSewram VGuruprasad KPNaidoo DBPhulukdaree AfalseWithania somnifera modulates cancer cachexia associated inflammatory cytokines and cell death in leukaemic THP-1 cells and peripheral blood mononuclear cells (PBMC's).<h4>Background</h4>Cancer and inflammation are associated with cachexia. Withania somnifera (W. somnifera) possesses antioxidant and anti-inflammatory potential. We investigated the potential of an aqueous extract of the root of W. somnifera (W_RE) to modulate cytokines, antioxidants and apoptosis in leukaemic THP-1 cells and peripheral blood mononuclear cells (PBMC's).<h4>Methods</h4>Cytotoxcity of W_RE was determined at 24 and 72 h (h). Oxidant scavenging activity of W_RE was evaluated (2, 2-diphenyl-1 picrylhydrazyl assay). Glutathione (GSH) levels, caspase (- 8, - 9, - 3/7) activities and adenosine triphosphate (ATP) levels (Luminometry) were thereafter assayed. Tumour necrosis factor-α (TNF-α), interleukin (IL)-6, IL-1β and IL-10 levels were also assessed2018-01-01T00:00:00Z2018 Apr2025-04-18T19:48:58.42Z2019-03-26T23:29:24ZS-EPMC58918972963158610.1186/s12906-018-2192-y