{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Tusi BK"],"funding":["NIDDK NIH HHS","NIGMS NIH HHS"],"pagination":["54-60"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC5899604"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["555(7694)"],"pubmed_abstract":["The formation of red blood cells begins with the differentiation of multipotent haematopoietic progenitors. Reconstructing the steps of this differentiation represents a general challenge in stem-cell biology. Here we used single-cell transcriptomics, fate assays and a theory that allows the prediction of cell fates from population snapshots to demonstrate that mouse haematopoietic progenitors differentiate through a continuous, hierarchical structure into seven blood lineages. We uncovered coupling between the erythroid and the basophil or mast cell fates, a global haematopoietic response to erythroid stress and novel growth factor receptors that regulate erythropoiesis. We defined a flow cytometry sorting strategy to purify early stages of erythroid differentiation, completely isolating classically defined burst-forming and colony-forming progenitors. We also found that the cell cycle is progressively remodelled during erythroid development and during a sharp transcriptional switch that ends the colony-forming progenitor stage and activates terminal differentiation. Our work showcases the utility of linking transcriptomic data to predictive fate models, and provides insights into lineage development in vivo."],"journal":["Nature"],"pubmed_title":["Population snapshots predict early haematopoietic and erythroid hierarchies."],"pmcid":["PMC5899604"],"funding_grant_id":["R01 DK100915","R01 DK099281","T32 GM080177"],"pubmed_authors":["Huh JR","Tusi BK","Wolock SL","Hidalgo D","Socolovsky M","Waisman A","Weinreb C","Zilionis R","Hwang Y","Klein AM"],"additional_accession":[]},"is_claimable":false,"name":"Population snapshots predict early haematopoietic and erythroid hierarchies.","description":"The formation of red blood cells begins with the differentiation of multipotent haematopoietic progenitors. Reconstructing the steps of this differentiation represents a general challenge in stem-cell biology. Here we used single-cell transcriptomics, fate assays and a theory that allows the prediction of cell fates from population snapshots to demonstrate that mouse haematopoietic progenitors differentiate through a continuous, hierarchical structure into seven blood lineages. We uncovered coupling between the erythroid and the basophil or mast cell fates, a global haematopoietic response to erythroid stress and novel growth factor receptors that regulate erythropoiesis. We defined a flow cytometry sorting strategy to purify early stages of erythroid differentiation, completely isolating classically defined burst-forming and colony-forming progenitors. We also found that the cell cycle is progressively remodelled during erythroid development and during a sharp transcriptional switch that ends the colony-forming progenitor stage and activates terminal differentiation. Our work showcases the utility of linking transcriptomic data to predictive fate models, and provides insights into lineage development in vivo.","dates":{"release":"2018-01-01T00:00:00Z","publication":"2018 Mar","modification":"2024-12-04T10:37:59.278Z","creation":"2019-03-26T23:51:50Z"},"accession":"S-EPMC5899604","cross_references":{"pubmed":["29466336"],"doi":["10.1038/nature25741"]}}