biostudies-literatureUnknown25(3)Tsuda B<h4>Background</h4>Historically, humoral immunity was considered unimportant in anti-tumor immunity, and the differentiation and anti-tumor activity of B cells in breast cancer are poorly understood. However, it was recently discovered that B cells participate in tumor immunity through both antibody production and immunosuppressive mechanisms. We analyzed the expression of B-cell differentiation markers in detail using fluorescence-activated cell sorting to investigate the relationship between B-cell subsets and breast cancer etiology.<h4>Methods</h4>Blood samples were taken from breast cancer patients and healthy donors, and peripheral blood mononuclear cells were collected. B cells at various stages of differentiation were identified by the expression of combinations of the cell surface Breast cancer (Tokyo, Japan)284-291https://www.ebi.ac.uk/biostudies/studies/S-EPMC5906508biostudies-literatureB-cell populations are expanded in breast cancer patients compared with healthy controls.PMC5906508Miyamoto AOgiya RTerao MSaito YYokoyama KKametani YOkamura TNiikura NTokuda YOshitanai RSuzuki YMorioka TMiyako HTsuda BfalseB-cell populations are expanded in breast cancer patients compared with healthy controls.<h4>Background</h4>Historically, humoral immunity was considered unimportant in anti-tumor immunity, and the differentiation and anti-tumor activity of B cells in breast cancer are poorly understood. However, it was recently discovered that B cells participate in tumor immunity through both antibody production and immunosuppressive mechanisms. We analyzed the expression of B-cell differentiation markers in detail using fluorescence-activated cell sorting to investigate the relationship between B-cell subsets and breast cancer etiology.<h4>Methods</h4>Blood samples were taken from breast cancer patients and healthy donors, and peripheral blood mononuclear cells were collected. B cells at various stages of differentiation were identified by the expression of combinations of the cell surface 2018-01-01T00:00:00Z2018 May2025-05-18T13:10:22.431Z2025-05-18T13:10:22.431ZS-EPMC59065082920484810.1007/s12282-017-0824-6