{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["8(1)"],"submitter":["Pourseif MM"],"pubmed_abstract":["<i><b>Introduction:</b></i> In this study, we targeted the worm stage of <i>Echinococcus granulosus</i> to design a novel multi-epitope B- and helper T-cell based vaccine construct for immunization of dogs against this multi-host parasite. <i><b>Methods:</b></i> The vaccine was designed based on the local Eg14-3-3 antigen (Ag). DNA samples were extracted from the protoscoleces of the infected sheep's liver, and then subjected to the polymerase chain reaction (PCR) with 14-3-3 specific forward and reverse primers. For the vaccine designing, several <i>in silico</i> steps were undertaken. Three-dimensional (3D) structure of the local Eg14-3-3 Ag was modeled by EasyModeller software. The protein modeling accuracy was then analyzed via various validation assays. Potential transmembrane helix, "],"journal":["BioImpacts : BI"],"pagination":["39-52"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC5915707"],"repository":["biostudies-literature"],"pubmed_title":["A novel B- and helper T-cell epitopes-based prophylactic vaccine against <i>Echinococcus granulosus</i>."],"pmcid":["PMC5915707"],"pubmed_authors":["Nematollahi A","Daghighkia H","Moghaddam G","Omidi Y","Pourseif MM"],"additional_accession":[]},"is_claimable":false,"name":"A novel B- and helper T-cell epitopes-based prophylactic vaccine against <i>Echinococcus granulosus</i>.","description":"<i><b>Introduction:</b></i> In this study, we targeted the worm stage of <i>Echinococcus granulosus</i> to design a novel multi-epitope B- and helper T-cell based vaccine construct for immunization of dogs against this multi-host parasite. <i><b>Methods:</b></i> The vaccine was designed based on the local Eg14-3-3 antigen (Ag). DNA samples were extracted from the protoscoleces of the infected sheep's liver, and then subjected to the polymerase chain reaction (PCR) with 14-3-3 specific forward and reverse primers. For the vaccine designing, several <i>in silico</i> steps were undertaken. Three-dimensional (3D) structure of the local Eg14-3-3 Ag was modeled by EasyModeller software. The protein modeling accuracy was then analyzed via various validation assays. Potential transmembrane helix, ","dates":{"release":"2018-01-01T00:00:00Z","publication":"2018","modification":"2026-05-01T01:55:09.378Z","creation":"2019-03-26T23:36:27Z"},"accession":"S-EPMC5915707","cross_references":{"pubmed":["29713601"],"doi":["10.15171/bi.2018.06"]}}