biostudies-literatureUnknown8(1)Pourseif MMIntroduction: In this study, we targeted the worm stage of Echinococcus granulosus to design a novel multi-epitope B- and helper T-cell based vaccine construct for immunization of dogs against this multi-host parasite. Methods: The vaccine was designed based on the local Eg14-3-3 antigen (Ag). DNA samples were extracted from the protoscoleces of the infected sheep's liver, and then subjected to the polymerase chain reaction (PCR) with 14-3-3 specific forward and reverse primers. For the vaccine designing, several in silico steps were undertaken. Three-dimensional (3D) structure of the local Eg14-3-3 Ag was modeled by EasyModeller software. The protein modeling accuracy was then analyzed via various validation assays. Potential transmembrane helix, BioImpacts : BI39-52https://www.ebi.ac.uk/biostudies/studies/S-EPMC5915707biostudies-literatureA novel B- and helper T-cell epitopes-based prophylactic vaccine against Echinococcus granulosus.PMC5915707Nematollahi ADaghighkia HMoghaddam GOmidi YPourseif MMfalseA novel B- and helper T-cell epitopes-based prophylactic vaccine against Echinococcus granulosus.Introduction: In this study, we targeted the worm stage of Echinococcus granulosus to design a novel multi-epitope B- and helper T-cell based vaccine construct for immunization of dogs against this multi-host parasite. Methods: The vaccine was designed based on the local Eg14-3-3 antigen (Ag). DNA samples were extracted from the protoscoleces of the infected sheep's liver, and then subjected to the polymerase chain reaction (PCR) with 14-3-3 specific forward and reverse primers. For the vaccine designing, several in silico steps were undertaken. Three-dimensional (3D) structure of the local Eg14-3-3 Ag was modeled by EasyModeller software. The protein modeling accuracy was then analyzed via various validation assays. Potential transmembrane helix, 2018-01-01T00:00:00Z20182026-05-01T01:55:09.378Z2019-03-26T23:36:27ZS-EPMC59157072971360110.15171/bi.2018.06