{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["10(3)"],"submitter":["Dong Q"],"funding":["DOC | National Institute of Standards and Technology (NIST)"],"pubmed_abstract":["We describe the creation of a mass spectral library composed of all identifiable spectra derived from the tryptic digest of the NISTmAb IgG1κ. The library is a unique reference spectral collection developed from over six million peptide-spectrum matches acquired by liquid chromatography-mass spectrometry (LC-MS) over a wide range of collision energy. Conventional one-dimensional (1D) LC-MS was used for various digestion conditions and 20- and 24-fraction two-dimensional (2D) LC-MS studies permitted in-depth analyses of single digests. Computer methods were developed for automated analysis of LC-MS isotopic clusters to determine the attributes for all ions detected in the 1D and 2D studies. The library contains a selection of over 12,600 high-quality tandem spectra of more than 3,300 peptid"],"journal":["mAbs"],"pagination":["354-369"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC5916550"],"repository":["biostudies-literature"],"pubmed_title":["The NISTmAb tryptic peptide spectral library for monoclonal antibody characterization."],"pmcid":["PMC5916550"],"pubmed_authors":["Stein SE","Bukhari TH","Liang Y","Markey SP","Tchekhovskoi DV","Dong Q","Mirokhin YA","Yan X"],"additional_accession":[]},"is_claimable":false,"name":"The NISTmAb tryptic peptide spectral library for monoclonal antibody characterization.","description":"We describe the creation of a mass spectral library composed of all identifiable spectra derived from the tryptic digest of the NISTmAb IgG1κ. The library is a unique reference spectral collection developed from over six million peptide-spectrum matches acquired by liquid chromatography-mass spectrometry (LC-MS) over a wide range of collision energy. Conventional one-dimensional (1D) LC-MS was used for various digestion conditions and 20- and 24-fraction two-dimensional (2D) LC-MS studies permitted in-depth analyses of single digests. Computer methods were developed for automated analysis of LC-MS isotopic clusters to determine the attributes for all ions detected in the 1D and 2D studies. The library contains a selection of over 12,600 high-quality tandem spectra of more than 3,300 peptid","dates":{"release":"2018-01-01T00:00:00Z","publication":"2018 Apr","modification":"2025-04-21T22:23:15.083Z","creation":"2019-03-26T23:33:42Z"},"accession":"S-EPMC5916550","cross_references":{"pubmed":["29425077"],"doi":["10.1080/19420862.2018.1436921"]}}