biostudies-literatureUnknown10(3)Dong QDOC | National Institute of Standards and Technology (NIST)We describe the creation of a mass spectral library composed of all identifiable spectra derived from the tryptic digest of the NISTmAb IgG1κ. The library is a unique reference spectral collection developed from over six million peptide-spectrum matches acquired by liquid chromatography-mass spectrometry (LC-MS) over a wide range of collision energy. Conventional one-dimensional (1D) LC-MS was used for various digestion conditions and 20- and 24-fraction two-dimensional (2D) LC-MS studies permitted in-depth analyses of single digests. Computer methods were developed for automated analysis of LC-MS isotopic clusters to determine the attributes for all ions detected in the 1D and 2D studies. The library contains a selection of over 12,600 high-quality tandem spectra of more than 3,300 peptidmAbs354-369https://www.ebi.ac.uk/biostudies/studies/S-EPMC5916550biostudies-literatureThe NISTmAb tryptic peptide spectral library for monoclonal antibody characterization.PMC5916550Stein SEBukhari THLiang YMarkey SPTchekhovskoi DVDong QMirokhin YAYan XfalseThe NISTmAb tryptic peptide spectral library for monoclonal antibody characterization.We describe the creation of a mass spectral library composed of all identifiable spectra derived from the tryptic digest of the NISTmAb IgG1κ. The library is a unique reference spectral collection developed from over six million peptide-spectrum matches acquired by liquid chromatography-mass spectrometry (LC-MS) over a wide range of collision energy. Conventional one-dimensional (1D) LC-MS was used for various digestion conditions and 20- and 24-fraction two-dimensional (2D) LC-MS studies permitted in-depth analyses of single digests. Computer methods were developed for automated analysis of LC-MS isotopic clusters to determine the attributes for all ions detected in the 1D and 2D studies. The library contains a selection of over 12,600 high-quality tandem spectra of more than 3,300 peptid2018-01-01T00:00:00Z2018 Apr2025-04-21T22:23:15.083Z2019-03-26T23:33:42ZS-EPMC59165502942507710.1080/19420862.2018.1436921