biostudies-literatureRong-Mullins XNHGRI NIH HHS607-619https://www.ebi.ac.uk/biostudies/studies/S-EPMC5919752biostudies-literatureUnknown8(2)Cellular metabolism can change the potency of a chemical's tumorigenicity. 4-nitroquinoline-1-oxide (4NQO) is a tumorigenic drug widely used on animal models for cancer research. Polymorphisms of the transcription factor Yrr1 confer different levels of resistance to 4NQO in <i>Saccharomyces cerevisiae</i> To study how different Yrr1 alleles regulate gene expression leading to resistance, transcriptomes of three isogenic <i>S</i><i>cerevisiae</i> strains carrying different Yrr1 alleles were profiled via RNA sequencing (RNA-Seq) and chromatin immunoprecipitation coupled with sequencing (ChIP-Seq) in the presence and absence of 4NQO. In response to 4NQO, all alleles of Yrr1 drove the expression of <i>SNQ2</i> (a multidrug transporter), which was highest in the presence of 4NQO resistance-confG3 (Bethesda, Md.)Transcriptional Profiling of <i>Saccharomyces cerevisiae</i> Reveals the Impact of Variation of a Single Transcription Factor on Differential Gene Expression in 4NQO, Fermentable, and Nonfermentable Carbon Sources.PMC5919752T32 HG000044Summers MRong-Mullins XAyers MCGallagher JEGfalseTranscriptional Profiling of <i>Saccharomyces cerevisiae</i> Reveals the Impact of Variation of a Single Transcription Factor on Differential Gene Expression in 4NQO, Fermentable, and Nonfermentable Carbon Sources.Cellular metabolism can change the potency of a chemical's tumorigenicity. 4-nitroquinoline-1-oxide (4NQO) is a tumorigenic drug widely used on animal models for cancer research. Polymorphisms of the transcription factor Yrr1 confer different levels of resistance to 4NQO in <i>Saccharomyces cerevisiae</i> To study how different Yrr1 alleles regulate gene expression leading to resistance, transcriptomes of three isogenic <i>S</i><i>cerevisiae</i> strains carrying different Yrr1 alleles were profiled via RNA sequencing (RNA-Seq) and chromatin immunoprecipitation coupled with sequencing (ChIP-Seq) in the presence and absence of 4NQO. In response to 4NQO, all alleles of Yrr1 drove the expression of <i>SNQ2</i> (a multidrug transporter), which was highest in the presence of 4NQO resistance-conf2018-01-01T00:00:00Z2018 Feb2026-05-01T19:45:10.363Z2019-03-26T23:33:47ZS-EPMC59197522920865010.1534/g3.117.300138