{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Pandey A"],"funding":["NIAID NIH HHS","National Natural Science Foundation of China"],"pagination":["103"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC5919948"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["8"],"pubmed_abstract":["<i>Brucella</i> spp. are intracellular vacuolar pathogens that causes brucellosis, a worldwide zoonosis of profound importance. We previously demonstrated that the activity of host unfolded protein response (UPR) sensor IRE1α (inositol-requiring enzyme 1) and ER-associated autophagy confer susceptibility to <i>Brucella melitensis</i> and <i>Brucella abortus</i> intracellular replication. However, the mechanism by which host IRE1α regulates the pathogen intracellular lifestyle remains elusive. In this study, by employing a diverse array of molecular approaches, including biochemical analyses, fluorescence microscopy imaging, and infection assays using primary cells derived from <i>Ern1</i> (encoding IRE1) conditional knockout mice, we address this gap in our understanding by demonstrating t"],"journal":["Frontiers in cellular and infection microbiology"],"pubmed_title":["Activation of Host IRE1α-Dependent Signaling Axis Contributes the Intracellular Parasitism of <i>Brucella melitensis</i>."],"pmcid":["PMC5919948"],"funding_grant_id":["R01 AI048496","81371773","U54 AI057156","R21 AI072446"],"pubmed_authors":["Cabello AL","Zhang MZ","de Figueiredo P","Qin QM","Feng X","Lin F","Feng HQ","Iwawaki T","Pandey A","Rice-Ficht A","Ficht TA","da Costa LF"],"additional_accession":[]},"is_claimable":false,"name":"Activation of Host IRE1α-Dependent Signaling Axis Contributes the Intracellular Parasitism of <i>Brucella melitensis</i>.","description":"<i>Brucella</i> spp. are intracellular vacuolar pathogens that causes brucellosis, a worldwide zoonosis of profound importance. We previously demonstrated that the activity of host unfolded protein response (UPR) sensor IRE1α (inositol-requiring enzyme 1) and ER-associated autophagy confer susceptibility to <i>Brucella melitensis</i> and <i>Brucella abortus</i> intracellular replication. However, the mechanism by which host IRE1α regulates the pathogen intracellular lifestyle remains elusive. In this study, by employing a diverse array of molecular approaches, including biochemical analyses, fluorescence microscopy imaging, and infection assays using primary cells derived from <i>Ern1</i> (encoding IRE1) conditional knockout mice, we address this gap in our understanding by demonstrating t","dates":{"release":"2018-01-01T00:00:00Z","publication":"2018","modification":"2025-05-31T22:41:28.15Z","creation":"2025-05-31T22:41:28.15Z"},"accession":"S-EPMC5919948","cross_references":{"pubmed":["29732320"],"doi":["10.3389/fcimb.2018.00103"]}}