biostudies-literatureUnknown9(28)Nesheim NChronic prostatitis/chronic pelvic pain syndrome (CP/CPPS) is associated with urinary tract symptoms and hormonal imbalances amongst others. The heterogeneous clinical presentation, unexplored molecular background and lack of prostate biopsies complicate therapy. Here, using liquid biopsies, we performed a comprehensive translational study on men diagnosed with CP/CPPS type III (<i>n</i><b><i>=</i></b> 50; median age 39.8, range 23-65) and age-matched controls (<i>n</i><b><i>=</i></b> 61; median age 36.8, range 20-69), considering biochemical parameters of blood and ejaculates, and epigenetic regulation of the estrogen receptor genes (<i>ESR1</i> and <i>ESR2</i>) in leukocytes isolated from blood (systemic regulation) and in somatic cells isolated from ejaculates (local regulation). We fouOncotarget19623-19639https://www.ebi.ac.uk/biostudies/studies/S-EPMC5929413biostudies-literatureElevated seminal plasma estradiol and epigenetic inactivation of <i>ESR1</i> and <i>ESR2</i> is associated with CP/CPPS.PMC5929413Pilatz AWeidner WSchuppe HCDansranjavin TBerg ESchagdarsurengin UEllem SSchambeck RRisbridger GNesheim NWagenlehner FHagenkotter CfalseElevated seminal plasma estradiol and epigenetic inactivation of <i>ESR1</i> and <i>ESR2</i> is associated with CP/CPPS.Chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS) is associated with urinary tract symptoms and hormonal imbalances amongst others. The heterogeneous clinical presentation, unexplored molecular background and lack of prostate biopsies complicate therapy. Here, using liquid biopsies, we performed a comprehensive translational study on men diagnosed with CP/CPPS type III (<i>n</i><b><i>=</i></b> 50; median age 39.8, range 23-65) and age-matched controls (<i>n</i><b><i>=</i></b> 61; median age 36.8, range 20-69), considering biochemical parameters of blood and ejaculates, and epigenetic regulation of the estrogen receptor genes (<i>ESR1</i> and <i>ESR2</i>) in leukocytes isolated from blood (systemic regulation) and in somatic cells isolated from ejaculates (local regulation). We fou2018-01-01T00:00:00Z2018 Apr2026-07-15T21:17:20.207Z2025-06-01T12:39:30.016ZS-EPMC59294132973197010.18632/oncotarget.24714