biostudies-literatureUlintz PJNCI NIH HHSAmerican Surgical Association Foundation (ASA Foundation)HHS | NIH | National Cancer Institute (NCI)2214-2224https://www.ebi.ac.uk/biostudies/studies/S-EPMC5932232biostudies-literatureUnknown24(9)<b>Purpose:</b> Recent studies have highlighted the existence of subclones in tumors. Lymph nodes are generally the first location of metastasis for most solid epithelial tumors, including colorectal cancer. We sought to understand the genetic origin of lymph node metastasis in colorectal cancer by evaluating the relationship between colorectal cancer subclones present in primary tumors and lymph nodes.<b>Experimental Design:</b> A total of 33 samples from seven colorectal cancers, including two or three spatially disparate regions from each primary tumor and one to four matched lymph nodes for each tumor, underwent next-generation whole-exome DNA sequencing, Affymetrix OncoScan SNP arrays, and targeted deep confirmatory sequencing. We performed mapping between SNPs and copy number events Clinical cancer research : an official journal of the American Association for Cancer ResearchLymph Node Metastases in Colon Cancer Are Polyclonal.PMC5932232R01CA0822235P50CA130810P30 CA046592L30 CA189003P30CA046592K08CA190645R01 CA082223P50 CA130810K08 CA190645Greenson JKWu RHardiman KMUlintz PJFearon ERfalseLymph Node Metastases in Colon Cancer Are Polyclonal.<b>Purpose:</b> Recent studies have highlighted the existence of subclones in tumors. Lymph nodes are generally the first location of metastasis for most solid epithelial tumors, including colorectal cancer. We sought to understand the genetic origin of lymph node metastasis in colorectal cancer by evaluating the relationship between colorectal cancer subclones present in primary tumors and lymph nodes.<b>Experimental Design:</b> A total of 33 samples from seven colorectal cancers, including two or three spatially disparate regions from each primary tumor and one to four matched lymph nodes for each tumor, underwent next-generation whole-exome DNA sequencing, Affymetrix OncoScan SNP arrays, and targeted deep confirmatory sequencing. We performed mapping between SNPs and copy number events 2018-01-01T00:00:00Z2018 May2025-04-03T23:13:18.536Z2019-06-06T22:51:37ZS-EPMC59322322920358910.1158/1078-0432.ccr-17-142510.1158/1078-0432.CCR-17-1425