biostudies-literatureVarghese RTNCATS NIH HHSNIDDK NIH HHSNational Institutes of Health549-555https://www.ebi.ac.uk/biostudies/studies/S-EPMC5946313biostudies-literatureUnknown20(3)<h4>Aims</h4>To compare the performance of population-based kinetics with that of directly measured C-peptide kinetics when used to calculate β-cell responsivity indices, and to study people with and without acute insulin resistance to ensure that population-based kinetics apply to all conditions where β-cell function is measured.<h4>Methods</h4>Somatostatin was used to inhibit endogenous insulin secretion in 56 people without diabetes. Subsequently, a C-peptide bolus was administered and the changing concentrations were used to calculate individual kinetic measures of C-peptide clearance. In addition, the participants were studied on 2 occasions in random order using an oral glucose tolerance test (OGTT). On one occasion, free fatty acid elevation, to cause insulin resistance, was achieveDiabetes, obesity & metabolismPerformance of individually measured vs population-based C-peptide kinetics to assess β-cell function in the presence and absence of acute insulin resistance.PMC59463135T32 DK007352-37T32 DK007352UL1 TR000135R01 DK078646R01 DK78646Shah MCobelli CDalla Man CSharma ALaurenti MCRizza RABailey KRVella AVarghese RTPiccinini FfalsePerformance of individually measured vs population-based C-peptide kinetics to assess β-cell function in the presence and absence of acute insulin resistance.<h4>Aims</h4>To compare the performance of population-based kinetics with that of directly measured C-peptide kinetics when used to calculate β-cell responsivity indices, and to study people with and without acute insulin resistance to ensure that population-based kinetics apply to all conditions where β-cell function is measured.<h4>Methods</h4>Somatostatin was used to inhibit endogenous insulin secretion in 56 people without diabetes. Subsequently, a C-peptide bolus was administered and the changing concentrations were used to calculate individual kinetic measures of C-peptide clearance. In addition, the participants were studied on 2 occasions in random order using an oral glucose tolerance test (OGTT). On one occasion, free fatty acid elevation, to cause insulin resistance, was achieve2018-01-01T00:00:00Z2018 Mar2025-04-05T15:06:59.757Z2019-06-06T19:29:39ZS-EPMC59463132886281210.1111/dom.13106