{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["15(Suppl 2)"],"submitter":["Xi S"],"pubmed_abstract":["Rationale: Whereas asbestos burden has been linked to cytogenetic alterations in malignant pleural mesotheliomas, epigenetic aberrations induced by these fibers have not been fully delineated. Objectives: The objective of this study was to establish an in vitro model to characterize early epigenetic events potentially contributing to malignant pleural mesothelioma. Methods: Normal human mesothelial cells (LP9 and LP3) were cultured with or without crocidolite asbestos fibers (1 or 2 μg/cm2) for up to 10 days. Messenger RNA, microRNA, and protein levels were assessed by quantitative reverse transcriptase–polymerase chain reaction and immunoblot techniques. Methylation-specific polymerase chain reaction, pyrosequencing, and quantitative chromatin immunoprecipitation techniques were used to c"],"journal":["Annals of the American Thoracic Society"],"pagination":["S123-S123"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC5946506"],"repository":["biostudies-literature"],"pubmed_title":["Asbestos Induces Epigenetic Repression of Ras Association Domain–Containing Protein 1, p16 Kinase 4a Inhibitor, and p14 Alternative Reading Frame in Normal Human Mesothelial Cells"],"pmcid":["PMC5946506"],"pubmed_authors":["Hoang C","Hong J","Ripley R","Reardon E","Zhang M","Schrump D","Payabyab E","Straughan D","Xi S"],"additional_accession":[]},"is_claimable":false,"name":"Asbestos Induces Epigenetic Repression of Ras Association Domain–Containing Protein 1, p16 Kinase 4a Inhibitor, and p14 Alternative Reading Frame in Normal Human Mesothelial Cells","description":"Rationale: Whereas asbestos burden has been linked to cytogenetic alterations in malignant pleural mesotheliomas, epigenetic aberrations induced by these fibers have not been fully delineated. Objectives: The objective of this study was to establish an in vitro model to characterize early epigenetic events potentially contributing to malignant pleural mesothelioma. Methods: Normal human mesothelial cells (LP9 and LP3) were cultured with or without crocidolite asbestos fibers (1 or 2 μg/cm2) for up to 10 days. Messenger RNA, microRNA, and protein levels were assessed by quantitative reverse transcriptase–polymerase chain reaction and immunoblot techniques. Methylation-specific polymerase chain reaction, pyrosequencing, and quantitative chromatin immunoprecipitation techniques were used to c","dates":{"release":"2018-01-01T00:00:00Z","publication":"2018 Apr","modification":"2025-04-22T09:54:29.018Z","creation":"2019-08-21T07:00:30Z"},"accession":"S-EPMC5946506","cross_references":{}}