biostudies-literatureUnknown15(Suppl 2)Xi SRationale: Whereas asbestos burden has been linked to cytogenetic alterations in malignant pleural mesotheliomas, epigenetic aberrations induced by these fibers have not been fully delineated. Objectives: The objective of this study was to establish an in vitro model to characterize early epigenetic events potentially contributing to malignant pleural mesothelioma. Methods: Normal human mesothelial cells (LP9 and LP3) were cultured with or without crocidolite asbestos fibers (1 or 2 μg/cm2) for up to 10 days. Messenger RNA, microRNA, and protein levels were assessed by quantitative reverse transcriptase–polymerase chain reaction and immunoblot techniques. Methylation-specific polymerase chain reaction, pyrosequencing, and quantitative chromatin immunoprecipitation techniques were used to cAnnals of the American Thoracic SocietyS123-S123https://www.ebi.ac.uk/biostudies/studies/S-EPMC5946506biostudies-literatureAsbestos Induces Epigenetic Repression of Ras Association Domain–Containing Protein 1, p16 Kinase 4a Inhibitor, and p14 Alternative Reading Frame in Normal Human Mesothelial CellsPMC5946506Hoang CHong JRipley RReardon EZhang MSchrump DPayabyab EStraughan DXi SfalseAsbestos Induces Epigenetic Repression of Ras Association Domain–Containing Protein 1, p16 Kinase 4a Inhibitor, and p14 Alternative Reading Frame in Normal Human Mesothelial CellsRationale: Whereas asbestos burden has been linked to cytogenetic alterations in malignant pleural mesotheliomas, epigenetic aberrations induced by these fibers have not been fully delineated. Objectives: The objective of this study was to establish an in vitro model to characterize early epigenetic events potentially contributing to malignant pleural mesothelioma. Methods: Normal human mesothelial cells (LP9 and LP3) were cultured with or without crocidolite asbestos fibers (1 or 2 μg/cm2) for up to 10 days. Messenger RNA, microRNA, and protein levels were assessed by quantitative reverse transcriptase–polymerase chain reaction and immunoblot techniques. Methylation-specific polymerase chain reaction, pyrosequencing, and quantitative chromatin immunoprecipitation techniques were used to c2018-01-01T00:00:00Z2018 Apr2025-04-22T09:54:29.018Z2019-08-21T07:00:30ZS-EPMC5946506