biostudies-literaturePelissier Vatter FASwiss National Science FoundationSchweizerischer Nationalfonds zur Förderung der Wissenschaftlichen ForschungEuropean Research CouncilNorges ForskningsrÃ¥dNIA NIH HHSNIDDK NIH HHSUniversitetet i BergenHelse VestSeventh Framework ProgrammeUniversität ZürichNCI NIH HHSNational Institutes of Health1205-1219https://www.ebi.ac.uk/biostudies/studies/S-EPMC5946804biostudies-literatureUnknown23(4)Aging is associated with tissue-level changes in cellular composition that are correlated with increased susceptibility to disease. Aging human mammary tissue shows skewed progenitor cell potency, resulting in diminished tumor-suppressive cell types and the accumulation of defective epithelial progenitors. Quantitative characterization of these age-emergent human cell subpopulations is lacking, impeding our understanding of the relationship between age and cancer susceptibility. We conducted single-cell resolution proteomic phenotyping of healthy breast epithelia from 57 women, aged 16-91 years, using mass cytometry. Remarkable heterogeneity was quantified within the two mammary epithelial lineages. Population partitioning identified a subset of aberrant basal-like luminal cells that accumCell reportsHigh-Dimensional Phenotyping Identifies Age-Emergent Cells in Human Mammary Epithelia.PMC5946804BC141351240130R00 AG033176316030-139220316030PP00P3-144874P30 CA033572R00AG033176336921BIOEF-17-001FK-74419-01-01223250911794R01AG040081144874UC4 DK108132FP/2007-2013R01 AG040081139220LaBarge MAStampfer MRBorowsky ADLee JKPelissier Vatter FASchapiro DBodenmiller BLorens JBChang HParvin BfalseHigh-Dimensional Phenotyping Identifies Age-Emergent Cells in Human Mammary Epithelia.Aging is associated with tissue-level changes in cellular composition that are correlated with increased susceptibility to disease. Aging human mammary tissue shows skewed progenitor cell potency, resulting in diminished tumor-suppressive cell types and the accumulation of defective epithelial progenitors. Quantitative characterization of these age-emergent human cell subpopulations is lacking, impeding our understanding of the relationship between age and cancer susceptibility. We conducted single-cell resolution proteomic phenotyping of healthy breast epithelia from 57 women, aged 16-91 years, using mass cytometry. Remarkable heterogeneity was quantified within the two mammary epithelial lineages. Population partitioning identified a subset of aberrant basal-like luminal cells that accum2018-01-01T00:00:00Z2018 Apr2026-05-06T06:37:10.02Z2019-03-26T23:37:18ZS-EPMC59468042969489610.1016/j.celrep.2018.03.114