biostudies-literatureShalaby KHIntramural NIH HHSNULL1229-1242.e6https://www.ebi.ac.uk/biostudies/studies/S-EPMC5951733biostudies-literatureUnknown142(4)<h4>Background</h4>Mechanisms that elicit mucosal T_H17 cell responses have been described, yet how these cells are sustained in chronically inflamed tissues remains unclear.<h4>Objective</h4>We sought to understand whether maintenance of lung T_H17 inflammation requires environmental agents in addition to antigen and to identify the lung antigen-presenting cell (APC) types that sustain the self-renewal of T_H17 cells.<h4>Methods</h4>Animals were exposed repeatedly to aspiration of ovalbumin alone or together with environmental adjuvants, including common house dust extract (HDE), to test their role in maintaining lung inflammation. Alternatively, antigen-specific effector/memory T_H17 cells, generated in culture with CD4⁺ T cells from Il1The Journal of allergy and clinical immunologyPathogenic T&lt;sub&gt;H&lt;/sub&gt;17 inflammation is sustained in the lungs by conventional dendritic cells and Toll-like receptor 4 signaling.PMC5951733ZIA ES102025-09Z01 ES102025ZIA ES102025Lyons-Cohen MRWhitehead GSShalaby KHPrinz ICook DNNakano HThomas SYfalsePathogenic T&lt;sub&gt;H&lt;/sub&gt;17 inflammation is sustained in the lungs by conventional dendritic cells and Toll-like receptor 4 signaling.<h4>Background</h4>Mechanisms that elicit mucosal T_H17 cell responses have been described, yet how these cells are sustained in chronically inflamed tissues remains unclear.<h4>Objective</h4>We sought to understand whether maintenance of lung T_H17 inflammation requires environmental agents in addition to antigen and to identify the lung antigen-presenting cell (APC) types that sustain the self-renewal of T_H17 cells.<h4>Methods</h4>Animals were exposed repeatedly to aspiration of ovalbumin alone or together with environmental adjuvants, including common house dust extract (HDE), to test their role in maintaining lung inflammation. Alternatively, antigen-specific effector/memory T_H17 cells, generated in culture with CD4⁺ T cells from Il12018-01-01T00:00:00Z2018 Oct2026-04-13T07:15:37.633Z2019-03-26T23:59:40ZS-EPMC59517332915495810.1016/j.jaci.2017.10.023