{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["9"],"submitter":["Fiedler MRM"],"pubmed_abstract":["In filamentous fungi, growth and protein secretion occurs predominantly at the tip of long, thread like cells termed hyphae. This requires coordinated regulation of multiple processes, including vesicle trafficking, exocytosis, and endocytosis, which are facilitated by a complex cytoskeletal apparatus. In this study, functional analyses of the small GTPase ArfA from <i>Aspergillus niger</i> demonstrate that this protein functionally complements the <i>Saccharomyces cerevisiae ARF1/2</i>, and that this protein is essential for <i>A. niger</i>. Loss-of-function and gain-of-function analyses demonstrate that titration of <i>arfA</i> expression impacts hyphal growth rate, hyphal tip morphology, and protein secretion. Moreover, localization of the endocytic machinery, visualized via fluorescent"],"journal":["Frontiers in microbiology"],"pagination":["878"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC5952172"],"repository":["biostudies-literature"],"pubmed_title":["Conditional Expression of the Small GTPase ArfA Impacts Secretion, Morphology, Growth, and Actin Ring Position in <i>Aspergillus niger</i>."],"pmcid":["PMC5952172"],"pubmed_authors":["Fiedler MRM","Kubisch C","Meyer V","Cairns TC","Koch O"],"additional_accession":[]},"is_claimable":false,"name":"Conditional Expression of the Small GTPase ArfA Impacts Secretion, Morphology, Growth, and Actin Ring Position in <i>Aspergillus niger</i>.","description":"In filamentous fungi, growth and protein secretion occurs predominantly at the tip of long, thread like cells termed hyphae. This requires coordinated regulation of multiple processes, including vesicle trafficking, exocytosis, and endocytosis, which are facilitated by a complex cytoskeletal apparatus. In this study, functional analyses of the small GTPase ArfA from <i>Aspergillus niger</i> demonstrate that this protein functionally complements the <i>Saccharomyces cerevisiae ARF1/2</i>, and that this protein is essential for <i>A. niger</i>. Loss-of-function and gain-of-function analyses demonstrate that titration of <i>arfA</i> expression impacts hyphal growth rate, hyphal tip morphology, and protein secretion. Moreover, localization of the endocytic machinery, visualized via fluorescent","dates":{"release":"2018-01-01T00:00:00Z","publication":"2018","modification":"2026-06-07T07:50:32.169Z","creation":"2019-03-26T23:40:28Z"},"accession":"S-EPMC5952172","cross_references":{"pubmed":["29867795"],"doi":["10.3389/fmicb.2018.00878"]}}