{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["13(5)"],"submitter":["Moran F"],"pubmed_abstract":["Grapevine Pinot gris virus (GPGV) is a widely distributed grapevine pathogen that has been associated to the grapevine leaf mottling and deformation disease. With the aim of better understanding the disease epidemiology and providing efficient control strategies a specific and quantitative duplex TaqMan real-time RT-PCR assay has been developed. This method has allowed reliable quantitation of the GPGV titer ranging from 30 up to 3 x 108 transcript copies, with a detection limit of 70 viral copies in plant material. The assay targets a grapevine internal control that reduces the occurrence of false negative results, thus increasing the diagnostic sensitivity of the technique. Viral isolates both associated and non-associated to symptoms from Greece, Slovakia and Spain have been successfull"],"journal":["PloS one"],"pagination":["e0197237"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC5953474"],"repository":["biostudies-literature"],"pubmed_title":["A novel specific duplex real-time RT-PCR method for absolute quantitation of Grapevine Pinot gris virus in plant material and single mites."],"pmcid":["PMC5953474"],"pubmed_authors":["Ruiz-Garcia AB","Glasa M","Predajna L","Lotos L","Katis N","Maliogka V","Moran F","Olmos A"],"additional_accession":[]},"is_claimable":false,"name":"A novel specific duplex real-time RT-PCR method for absolute quantitation of Grapevine Pinot gris virus in plant material and single mites.","description":"Grapevine Pinot gris virus (GPGV) is a widely distributed grapevine pathogen that has been associated to the grapevine leaf mottling and deformation disease. With the aim of better understanding the disease epidemiology and providing efficient control strategies a specific and quantitative duplex TaqMan real-time RT-PCR assay has been developed. This method has allowed reliable quantitation of the GPGV titer ranging from 30 up to 3 x 108 transcript copies, with a detection limit of 70 viral copies in plant material. The assay targets a grapevine internal control that reduces the occurrence of false negative results, thus increasing the diagnostic sensitivity of the technique. Viral isolates both associated and non-associated to symptoms from Greece, Slovakia and Spain have been successfull","dates":{"release":"2018-01-01T00:00:00Z","publication":"2018","modification":"2025-04-04T09:50:22.822Z","creation":"2019-03-26T23:39:09Z"},"accession":"S-EPMC5953474","cross_references":{"pubmed":["29763449"],"doi":["10.1371/journal.pone.0197237"]}}