biostudies-literaturePeng XAdelson Medical Research FoundationNCI NIH HHSNatural Scientific Foundation of ChinaCCSGNIHNIH HHSCancer Prevention and Research Institute of TexasMD Anderson Cancer CenterLorraine Dell Program in Bioinformatics for Personalization of Cancer Medicine817-828.e7https://www.ebi.ac.uk/biostudies/studies/S-EPMC5953833biostudies-literatureUnknown33(5)Adenosine (A) to inosine (I) RNA editing introduces many nucleotide changes in cancer transcriptomes. However, due to the complexity of post-transcriptional regulation, the contribution of RNA editing to proteomic diversity in human cancers remains unclear. Here, we performed an integrated analysis of TCGA genomic data and CPTAC proteomic data. Despite limited site diversity, we demonstrate that A-to-I RNA editing contributes to proteomic diversity in breast cancer through changes in amino acid sequences. We validate the presence of editing events at both RNA and protein levels. The edited COPA protein increases proliferation, migration, and invasion of cancer cells in vitro. Our study suggests an important contribution of A-to-I RNA editing to protein diversity in cancer and highlights itCancer cellA-to-I RNA Editing Contributes to Proteomic Diversity in Cancer.PMC5953833CA175486P50 CA098258S10 OD012304CA20985181572777CA016672RP140462R01 CA175486CA168394CA098258U01 CA168394P30 CA016672R50 CA221675U24 CA209851RP130397CA1438831S10OD012304-01U01 CA217842U24 CA143883RR150085Peng XJeong KJYu SHan LLabrie MTsang YHLu YMojumdar KZhang MScott KLZhou ZLiang HHawke DHHwu PXu XWang YMills GBfalseA-to-I RNA Editing Contributes to Proteomic Diversity in Cancer.Adenosine (A) to inosine (I) RNA editing introduces many nucleotide changes in cancer transcriptomes. However, due to the complexity of post-transcriptional regulation, the contribution of RNA editing to proteomic diversity in human cancers remains unclear. Here, we performed an integrated analysis of TCGA genomic data and CPTAC proteomic data. Despite limited site diversity, we demonstrate that A-to-I RNA editing contributes to proteomic diversity in breast cancer through changes in amino acid sequences. We validate the presence of editing events at both RNA and protein levels. The edited COPA protein increases proliferation, migration, and invasion of cancer cells in vitro. Our study suggests an important contribution of A-to-I RNA editing to protein diversity in cancer and highlights it2018-01-01T00:00:00Z2018 May2026-05-02T02:27:13.647Z2019-06-06T23:03:39ZS-EPMC59538332970645410.1016/j.ccell.2018.03.026