biostudies-literatureUnknown122(10)Zhang Y<h4>Rationale</h4>Ca²⁺ homeostasis-a critical determinant of cardiac contractile function-is critically regulated by SERCA2a (sarcoplasmic reticulum Ca²⁺-ATPase 2a). Our previous study has identified <i>ZFAS1</i> as a new lncRNA biomarker of acute myocardial infarction (MI).<h4>Objective</h4>To evaluate the effects of <i>ZFAS1</i> on SERCA2a and the associated Ca²⁺ homeostasis and cardiac contractile function in the setting of MI.<h4>Methods and results</h4><i>ZFAS1</i> expression was robustly increased in cytoplasm and sarcoplasmic reticulum in a mouse model of MI and a cellular model of hypoxia. Knockdown of endogenous <i>ZFAS1</i> by virus-mediated silencing shRNA partially abrogated the ischemia-induced contractile dysfunction. Overexpression of <i>ZFASCirculation research1354-1368https://www.ebi.ac.uk/biostudies/studies/S-EPMC5959220biostudies-literatureLncRNA <i>ZFAS1</i> as a SERCA2a Inhibitor to Cause Intracellular Ca²⁺ Overload and Contractile Dysfunction in a Mouse Model of Myocardial Infarction.PMC5959220Jiao LPan ZLi CLu YXu CLi QYang BLi MZhuang YSun LLi YShao YZhang YGao YYang RXuan LfalseLncRNA <i>ZFAS1</i> as a SERCA2a Inhibitor to Cause Intracellular Ca²⁺ Overload and Contractile Dysfunction in a Mouse Model of Myocardial Infarction.<h4>Rationale</h4>Ca²⁺ homeostasis-a critical determinant of cardiac contractile function-is critically regulated by SERCA2a (sarcoplasmic reticulum Ca²⁺-ATPase 2a). Our previous study has identified <i>ZFAS1</i> as a new lncRNA biomarker of acute myocardial infarction (MI).<h4>Objective</h4>To evaluate the effects of <i>ZFAS1</i> on SERCA2a and the associated Ca²⁺ homeostasis and cardiac contractile function in the setting of MI.<h4>Methods and results</h4><i>ZFAS1</i> expression was robustly increased in cytoplasm and sarcoplasmic reticulum in a mouse model of MI and a cellular model of hypoxia. Knockdown of endogenous <i>ZFAS1</i> by virus-mediated silencing shRNA partially abrogated the ischemia-induced contractile dysfunction. Overexpression of <i>ZFAS2018-01-01T00:00:00Z2018 May2026-06-14T06:12:37.481Z2026-06-14T03:16:08.79ZS-EPMC59592202947598210.1161/CIRCRESAHA.117.312117