biostudies-literatureOnizuka KNIGMS NIH HHS2191-2199https://www.ebi.ac.uk/biostudies/studies/S-EPMC5969911biostudies-literatureUnknown25(7)A variety of enzymes have been found to interact with double-stranded RNA (dsRNA) in order to carry out its functions. We have endeavored to prepare the covalently crosslinked native-like duplex RNA, which could be useful for biochemical studies and RNA nanotechnology. In this study, the interstrand covalently linked duplex RNA was formed by a crosslinking reaction between vinylpurine (VP) and the target cytosine or uracil in RNA. We measured melting temperatures and CD spectra to identify the properties of the VP crosslinked duplex RNA. The crosslinking formation increased the thermodynamic stability without disturbing the natural conformation of dsRNA. In addition, a competitive binding experiment with the duplex RNA binding enzyme, ADAR2, showed the crosslinked dsRNA bound the protein wBioorganic & medicinal chemistrySynthesis of native-like crosslinked duplex RNA and study of its properties.PMC5969911R01 GM061115Monteleone LRImoto SThomas JMOnizuka KHazemi MEBeal PAYamada KNagatsugi FfalseSynthesis of native-like crosslinked duplex RNA and study of its properties.A variety of enzymes have been found to interact with double-stranded RNA (dsRNA) in order to carry out its functions. We have endeavored to prepare the covalently crosslinked native-like duplex RNA, which could be useful for biochemical studies and RNA nanotechnology. In this study, the interstrand covalently linked duplex RNA was formed by a crosslinking reaction between vinylpurine (VP) and the target cytosine or uracil in RNA. We measured melting temperatures and CD spectra to identify the properties of the VP crosslinked duplex RNA. The crosslinking formation increased the thermodynamic stability without disturbing the natural conformation of dsRNA. In addition, a competitive binding experiment with the duplex RNA binding enzyme, ADAR2, showed the crosslinked dsRNA bound the protein w2017-01-01T00:00:00Z2017 Apr2025-04-04T01:26:49.887Z2019-03-26T23:38:35ZS-EPMC59699112826805210.1016/j.bmc.2017.02.034