biostudies-literatureLei LGouvernement du Canada | Natural Sciences and Engineering Research Council of CanadaResearch ManitobaGouvernement du Canada | Canadian Institutes of Health Researche00651-17https://www.ebi.ac.uk/biostudies/studies/S-EPMC5974433biostudies-literatureUnknown38(12)The pituitary-derived somatolactotrophe GH₃ cells secrete both growth hormone (GH) and prolactin (PRL). We have found that the hnRNP L and L-like (LL) paralogs differentially regulate alternative splicing of genes in these cells. Here, we show that hnRNP L is essential for PRL only, but LL is essential for both PRL and GH production. Transcriptome-wide RNA sequencing (RNA-Seq) analysis indicates that they differentially control groups of hormone or hormone-related genes involved in hormone production/regulation at total transcript and alternative exon levels. Interestingly, hnRNP L also specifically binds and prevents the aberrant usage of a nonconserved CA-rich intron piece of <i>Prl</i> pre-mRNA transcripts, and many others involved in endocrine functions, to prevent mostly crMolecular and cellular biologyMultilevel Differential Control of Hormone Gene Expression Programs by hnRNP L and LL in Pituitary Cells.PMC5974433RGPIN-2016-06004FRN106608Chair FundSohail MLei LLiu LCao WDas UXie JChen YWu YLiu GfalseMultilevel Differential Control of Hormone Gene Expression Programs by hnRNP L and LL in Pituitary Cells.The pituitary-derived somatolactotrophe GH₃ cells secrete both growth hormone (GH) and prolactin (PRL). We have found that the hnRNP L and L-like (LL) paralogs differentially regulate alternative splicing of genes in these cells. Here, we show that hnRNP L is essential for PRL only, but LL is essential for both PRL and GH production. Transcriptome-wide RNA sequencing (RNA-Seq) analysis indicates that they differentially control groups of hormone or hormone-related genes involved in hormone production/regulation at total transcript and alternative exon levels. Interestingly, hnRNP L also specifically binds and prevents the aberrant usage of a nonconserved CA-rich intron piece of <i>Prl</i> pre-mRNA transcripts, and many others involved in endocrine functions, to prevent mostly cr2018-01-01T00:00:00Z2018 Jun2026-04-30T09:48:59.985Z2019-03-26T23:39:37ZS-EPMC59744332961015110.1128/MCB.00651-17