biostudies-literatureLadowski JMNIAID NIH HHSNIAMS NIH HHSNIGMS NIH HHS2957-2964https://www.ebi.ac.uk/biostudies/studies/S-EPMC5978423biostudies-literatureUnknown200(8)Genetically engineered pig organs could provide transplants to all patients with end-stage organ failure, but Ab-mediated rejection remains an issue. This study examines the class II swine leukocyte Ag (SLA) as a target of epitope-restricted Ab binding. Transfection of individual α- and β-chains into human embryonic kidney cells resulted in both traditional and hybrid class II SLA molecules. Sera from individuals on the solid organ transplant waiting list were tested for Ab binding and cytotoxicity to this panel of class II SLA single-Ag cells. A series of elution studies from an SLA-DQ cell line were performed. Our results indicate that human sera contain Abs specific for and cytotoxic against class II SLA. Our elution studies revealed that sera bind the SLA-DQ molecule in an epitope-restJournal of immunology (Baltimore, Md. : 1950)Examining the Biosynthesis and Xenoantigenicity of Class II Swine Leukocyte Antigen Proteins.PMC5978423P30 AR048311P30 AI027767T32 GM008361Hauptfeld-Dolejsek VTector AJEckhoff DELadowski JMReyes LMTector MMartens GRWang ZYfalseExamining the Biosynthesis and Xenoantigenicity of Class II Swine Leukocyte Antigen Proteins.Genetically engineered pig organs could provide transplants to all patients with end-stage organ failure, but Ab-mediated rejection remains an issue. This study examines the class II swine leukocyte Ag (SLA) as a target of epitope-restricted Ab binding. Transfection of individual α- and β-chains into human embryonic kidney cells resulted in both traditional and hybrid class II SLA molecules. Sera from individuals on the solid organ transplant waiting list were tested for Ab binding and cytotoxicity to this panel of class II SLA single-Ag cells. A series of elution studies from an SLA-DQ cell line were performed. Our results indicate that human sera contain Abs specific for and cytotoxic against class II SLA. Our elution studies revealed that sera bind the SLA-DQ molecule in an epitope-rest2018-01-01T00:00:00Z2018 Apr2026-05-04T17:31:12.39Z2025-05-29T20:45:31.154ZS-EPMC59784232954057910.4049/jimmunol.1800022