{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Aghajanova L"],"funding":["Eunice Kennedy Shriver National Institute of Child Health and Human Development","NICHD NIH HHS","IntegraMed Fertility"],"pagination":["757-770"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC5984879"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["35(5)"],"pubmed_abstract":["PURPOSE:The study aims to test the hypothesis that platelet-rich plasma (PRP) stimulates cellular processes involved in endometrial regeneration relevant to clinical management of poor endometrial growth or intrauterine scarring. METHODS:Human endometrial stromal fibroblasts (eSF), endometrial mesenchymal stem cells (eMSC), bone marrow-derived mesenchymal stem cells (BM-MSC), and Ishikawa endometrial adenocarcinoma cells (IC) were cultured with/without 5% activated (a) PRP, non-activated (na) PRP, aPPP (platelet-poor-plasma), and naPPP. Treatment effects were evaluated with cell proliferation (WST-1), wound healing, and chemotaxis Transwell migration assays. Mesenchymal-to-epithelial transition (MET) was evaluated by cytokeratin and vimentin expression. Differential gene expression of vari"],"journal":["Journal of assisted reproduction and genetics"],"pubmed_title":["In vitro evidence that platelet-rich plasma stimulates cellular processes involved in endometrial regeneration."],"pmcid":["PMC5984879"],"funding_grant_id":["R24 HD102061","P50HD055764","U54 HD055764","P50 HD055764"],"pubmed_authors":["Houshdaran S","Balayan S","Irwin JC","Aghajanova L","Huddleston HG","Giudice LC","Manvelyan E"],"additional_accession":[]},"is_claimable":false,"name":"In vitro evidence that platelet-rich plasma stimulates cellular processes involved in endometrial regeneration.","description":"PURPOSE:The study aims to test the hypothesis that platelet-rich plasma (PRP) stimulates cellular processes involved in endometrial regeneration relevant to clinical management of poor endometrial growth or intrauterine scarring. METHODS:Human endometrial stromal fibroblasts (eSF), endometrial mesenchymal stem cells (eMSC), bone marrow-derived mesenchymal stem cells (BM-MSC), and Ishikawa endometrial adenocarcinoma cells (IC) were cultured with/without 5% activated (a) PRP, non-activated (na) PRP, aPPP (platelet-poor-plasma), and naPPP. Treatment effects were evaluated with cell proliferation (WST-1), wound healing, and chemotaxis Transwell migration assays. Mesenchymal-to-epithelial transition (MET) was evaluated by cytokeratin and vimentin expression. Differential gene expression of vari","dates":{"release":"2018-01-01T00:00:00Z","publication":"2018 May","modification":"2025-04-19T05:32:56.441Z","creation":"2019-06-06T22:52:20Z"},"accession":"S-EPMC5984879","cross_references":{"pubmed":["29404863"],"doi":["10.1007/s10815-018-1130-8"]}}