biostudies-literatureAghajanova LEunice Kennedy Shriver National Institute of Child Health and Human DevelopmentNICHD NIH HHSIntegraMed Fertility757-770https://www.ebi.ac.uk/biostudies/studies/S-EPMC5984879biostudies-literatureUnknown35(5)PURPOSE:The study aims to test the hypothesis that platelet-rich plasma (PRP) stimulates cellular processes involved in endometrial regeneration relevant to clinical management of poor endometrial growth or intrauterine scarring. METHODS:Human endometrial stromal fibroblasts (eSF), endometrial mesenchymal stem cells (eMSC), bone marrow-derived mesenchymal stem cells (BM-MSC), and Ishikawa endometrial adenocarcinoma cells (IC) were cultured with/without 5% activated (a) PRP, non-activated (na) PRP, aPPP (platelet-poor-plasma), and naPPP. Treatment effects were evaluated with cell proliferation (WST-1), wound healing, and chemotaxis Transwell migration assays. Mesenchymal-to-epithelial transition (MET) was evaluated by cytokeratin and vimentin expression. Differential gene expression of variJournal of assisted reproduction and geneticsIn vitro evidence that platelet-rich plasma stimulates cellular processes involved in endometrial regeneration.PMC5984879R24 HD102061P50HD055764U54 HD055764P50 HD055764Houshdaran SBalayan SIrwin JCAghajanova LHuddleston HGGiudice LCManvelyan EfalseIn vitro evidence that platelet-rich plasma stimulates cellular processes involved in endometrial regeneration.PURPOSE:The study aims to test the hypothesis that platelet-rich plasma (PRP) stimulates cellular processes involved in endometrial regeneration relevant to clinical management of poor endometrial growth or intrauterine scarring. METHODS:Human endometrial stromal fibroblasts (eSF), endometrial mesenchymal stem cells (eMSC), bone marrow-derived mesenchymal stem cells (BM-MSC), and Ishikawa endometrial adenocarcinoma cells (IC) were cultured with/without 5% activated (a) PRP, non-activated (na) PRP, aPPP (platelet-poor-plasma), and naPPP. Treatment effects were evaluated with cell proliferation (WST-1), wound healing, and chemotaxis Transwell migration assays. Mesenchymal-to-epithelial transition (MET) was evaluated by cytokeratin and vimentin expression. Differential gene expression of vari2018-01-01T00:00:00Z2018 May2025-04-19T05:32:56.441Z2019-06-06T22:52:20ZS-EPMC59848792940486310.1007/s10815-018-1130-8