{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Zhu Z"],"funding":["Natural Science Foundation of Liaoning Province","National Natural Science Foundation of China"],"pagination":["156"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC5989348"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["11"],"pubmed_abstract":["<h4>Background</h4>The synchronous triacylglycerol (TAG) production with the growth is a key step to lower the cost of the microalgae-based biofuel production. Phospholipid: diacylglycerol acyltransferase (PDAT) has been identified recently and catalyzes the phospholipid contributing acyl group to diacylglycerol to synthesize TAG, and is considered as the important source of TAG in <i>Chlamydomonas reinhardtii</i>.<h4>Results</h4>Using a chimeric Hsp70A-RbcS2 promoter, exogenous PDAT form <i>Saccharomyces cerevisiae</i> fused with a chloroplast transit peptide was expressed in <i>C. reinhardtii</i> CC-137. Proved by western blot, the expression of ScPDAT showed a synchronous trend to the growth in the exponential phase. Compared to the wild type, the strain of <i>Scpdat</i> achieved 22% in"],"journal":["Biotechnology for biofuels"],"pubmed_title":["The synchronous TAG production with the growth by the expression of chloroplast transit peptide-fused ScPDAT in <i>Chlamydomonas reinhardtii</i>."],"pmcid":["PMC5989348"],"funding_grant_id":["2014020090","31470432"],"pubmed_authors":["Yin Y","Tian J","Liu Y","Liu J","Fan X","Zhu Z","Fan Y","Cao X","Xue S","Yang M","Yuan G"],"additional_accession":[]},"is_claimable":false,"name":"The synchronous TAG production with the growth by the expression of chloroplast transit peptide-fused ScPDAT in <i>Chlamydomonas reinhardtii</i>.","description":"<h4>Background</h4>The synchronous triacylglycerol (TAG) production with the growth is a key step to lower the cost of the microalgae-based biofuel production. Phospholipid: diacylglycerol acyltransferase (PDAT) has been identified recently and catalyzes the phospholipid contributing acyl group to diacylglycerol to synthesize TAG, and is considered as the important source of TAG in <i>Chlamydomonas reinhardtii</i>.<h4>Results</h4>Using a chimeric Hsp70A-RbcS2 promoter, exogenous PDAT form <i>Saccharomyces cerevisiae</i> fused with a chloroplast transit peptide was expressed in <i>C. reinhardtii</i> CC-137. Proved by western blot, the expression of ScPDAT showed a synchronous trend to the growth in the exponential phase. Compared to the wild type, the strain of <i>Scpdat</i> achieved 22% in","dates":{"release":"2018-01-01T00:00:00Z","publication":"2018","modification":"2026-04-07T20:11:14.575Z","creation":"2019-03-26T23:42:11Z"},"accession":"S-EPMC5989348","cross_references":{"pubmed":["29928307"],"doi":["10.1186/s13068-018-1160-6"]}}