biostudies-literatureZhu ZNatural Science Foundation of Liaoning ProvinceNational Natural Science Foundation of China156https://www.ebi.ac.uk/biostudies/studies/S-EPMC5989348biostudies-literatureUnknown11<h4>Background</h4>The synchronous triacylglycerol (TAG) production with the growth is a key step to lower the cost of the microalgae-based biofuel production. Phospholipid: diacylglycerol acyltransferase (PDAT) has been identified recently and catalyzes the phospholipid contributing acyl group to diacylglycerol to synthesize TAG, and is considered as the important source of TAG in <i>Chlamydomonas reinhardtii</i>.<h4>Results</h4>Using a chimeric Hsp70A-RbcS2 promoter, exogenous PDAT form <i>Saccharomyces cerevisiae</i> fused with a chloroplast transit peptide was expressed in <i>C. reinhardtii</i> CC-137. Proved by western blot, the expression of ScPDAT showed a synchronous trend to the growth in the exponential phase. Compared to the wild type, the strain of <i>Scpdat</i> achieved 22% inBiotechnology for biofuelsThe synchronous TAG production with the growth by the expression of chloroplast transit peptide-fused ScPDAT in <i>Chlamydomonas reinhardtii</i>.PMC5989348201402009031470432Yin YTian JLiu YLiu JFan XZhu ZFan YCao XXue SYang MYuan GfalseThe synchronous TAG production with the growth by the expression of chloroplast transit peptide-fused ScPDAT in <i>Chlamydomonas reinhardtii</i>.<h4>Background</h4>The synchronous triacylglycerol (TAG) production with the growth is a key step to lower the cost of the microalgae-based biofuel production. Phospholipid: diacylglycerol acyltransferase (PDAT) has been identified recently and catalyzes the phospholipid contributing acyl group to diacylglycerol to synthesize TAG, and is considered as the important source of TAG in <i>Chlamydomonas reinhardtii</i>.<h4>Results</h4>Using a chimeric Hsp70A-RbcS2 promoter, exogenous PDAT form <i>Saccharomyces cerevisiae</i> fused with a chloroplast transit peptide was expressed in <i>C. reinhardtii</i> CC-137. Proved by western blot, the expression of ScPDAT showed a synchronous trend to the growth in the exponential phase. Compared to the wild type, the strain of <i>Scpdat</i> achieved 22% in2018-01-01T00:00:00Z20182026-04-07T20:11:14.575Z2019-03-26T23:42:11ZS-EPMC59893482992830710.1186/s13068-018-1160-6