biostudies-literature00520Zhang XNCATS NIH HHSNCRR NIH HHSNIAID NIH HHSNIEHS NIH HHSNHGRI NIH HHSNINDS NIH HHSNIAMS NIH HHS629-641https://www.ebi.ac.uk/biostudies/studies/S-EPMC5992571biostudies-literatureUnknown10(5)AIM:We aim to study DNA methylation (DNAm) variations associated with childhood asthma. METHODS:Nasal DNAm was compared between sibling pairs discordant for asthma, 29 sib pairs for genome-wide association studies and 54 sib pairs for verification by pyrosequencing. Associations of methylation with asthma symptoms, allergy and environmental exposures were evaluated. In vitro experiments and functional genomic analyses were performed to explore biologic relevance. RESULTS:Three CpGs were associated with asthma. cg14830002 was associated with allergies in nonasthmatics. cg23602092 was associated with asthma symptoms. cg14830002 and cg23602092 were associated with traffic-related air pollution exposure. Nearby genes were transcriptionally regulated by diesel exhaust, house dust mite and 5-aza-2'-deoxycytidine. Active chromatin marks and transcription factor binding were found around these sites. CONCLUSION:We identified novel DNAm variations associated with childhood asthma and suggested new disease-contributing epigenetic mechanisms.EpigenomicsNasal DNA methylation is associated with childhood asthma.PMC5992571R21 HG008186UL1 RR026314R01 NS099068UL1 TR001425P30 ES006096R21 AI101375P30 AR070549R21 AI119236Burleson JDBaker TAJi HBryan KSZhang XUlm AWeirauch MTBiagini Myers JMChen XButsch Kovacic MS52falseNasal DNA methylation is associated with childhood asthma.AIM:We aim to study DNA methylation (DNAm) variations associated with childhood asthma. METHODS:Nasal DNAm was compared between sibling pairs discordant for asthma, 29 sib pairs for genome-wide association studies and 54 sib pairs for verification by pyrosequencing. Associations of methylation with asthma symptoms, allergy and environmental exposures were evaluated. In vitro experiments and functional genomic analyses were performed to explore biologic relevance. RESULTS:Three CpGs were associated with asthma. cg14830002 was associated with allergies in nonasthmatics. cg23602092 was associated with asthma symptoms. cg14830002 and cg23602092 were associated with traffic-related air pollution exposure. Nearby genes were transcriptionally regulated by diesel exhaust, house dust mite and 5-aza-2'-deoxycytidine. Active chromatin marks and transcription factor binding were found around these sites. CONCLUSION:We identified novel DNAm variations associated with childhood asthma and suggested new disease-contributing epigenetic mechanisms.2018-01-01T00:00:00Z2018 May2024-11-05T22:55:38.991Z2019-06-06T22:52:27ZS-EPMC59925712969219810.2217/epi-2017-0127