{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Treen N"],"funding":["NHGRI","NHGRI NIH HHS","NINDS NIH HHS","NIH"],"pagination":["1150-1156.e4"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC5996753"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["28(7)"],"pubmed_abstract":["Most animal embryos display a delay in the activation of zygotic transcription during early embryogenesis [1]. This process is thought to help coordinate rapid increases in cell number during early development [2]. The timing of zygotic genome activation (ZGA) during the maternal-to-zygotic transition (MZT) remains uncertain despite extensive efforts. We explore ZGA in the simple protovertebrate, Ciona intestinalis. Single-cell RNA sequencing (RNA-seq) assays identified Cyclin B3 (Ccnb3) as a putative mediator of ZGA. Maternal Ccnb3 transcripts rapidly diminish in abundance during the onset of zygotic transcription at the 8-cell and 16-cell stages. Disruption of Ccnb3 activity results in precocious activation of zygotic transcription, while overexpression abolishes normal activation. These"],"journal":["Current biology : CB"],"pubmed_title":["Depletion of Maternal Cyclin B3 Contributes to Zygotic Genome Activation in the Ciona Embryo."],"pmcid":["PMC5996753"],"funding_grant_id":["NS076542","T32HG003284","T32 HG003284","R01 NS076542"],"pubmed_authors":["Levine M","Heist T","Wang W","Treen N"],"additional_accession":[]},"is_claimable":false,"name":"Depletion of Maternal Cyclin B3 Contributes to Zygotic Genome Activation in the Ciona Embryo.","description":"Most animal embryos display a delay in the activation of zygotic transcription during early embryogenesis [1]. This process is thought to help coordinate rapid increases in cell number during early development [2]. The timing of zygotic genome activation (ZGA) during the maternal-to-zygotic transition (MZT) remains uncertain despite extensive efforts. We explore ZGA in the simple protovertebrate, Ciona intestinalis. Single-cell RNA sequencing (RNA-seq) assays identified Cyclin B3 (Ccnb3) as a putative mediator of ZGA. Maternal Ccnb3 transcripts rapidly diminish in abundance during the onset of zygotic transcription at the 8-cell and 16-cell stages. Disruption of Ccnb3 activity results in precocious activation of zygotic transcription, while overexpression abolishes normal activation. These","dates":{"release":"2018-01-01T00:00:00Z","publication":"2018 Apr","modification":"2025-04-03T22:20:42.115Z","creation":"2019-08-04T08:11:39Z"},"accession":"S-EPMC5996753","cross_references":{"pubmed":["29576477"],"doi":["10.1016/j.cub.2018.02.046"]}}