{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Hillman EM"],"funding":["Simons Foundation Collaboration on the Global Brain","NEI NIH HHS","Kavli Institute for Brain Science","NHLBI NIH HHS","Coulter Foundation","Columbia-Coulter Translational Research Partnership","NINDS NIH HHS","Department of Defense MURI","National Institutes of Health","National Science Foundation"],"pagination":["190-200"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC6002850"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["50"],"pubmed_abstract":["As optical reporters and modulators of cellular activity have become increasingly sophisticated, the amount that can be learned about the brain via high-speed cellular imaging has increased dramatically. However, despite fervent innovation, point-scanning microscopy is facing a fundamental limit in achievable 3D imaging speeds and fields of view. A range of alternative approaches are emerging, some of which are moving away from point-scanning to use axially-extended beams or sheets of light, for example swept confocally aligned planar excitation (SCAPE) microscopy. These methods are proving effective for high-speed volumetric imaging of the nervous system of small organisms such as Drosophila (fruit fly) and D. Rerio (Zebrafish), and are showing promise for imaging activity in the living m"],"journal":["Current opinion in neurobiology"],"pubmed_title":["High-speed 3D imaging of cellular activity in the brain using axially-extended beams and light sheets."],"pmcid":["PMC6002850"],"funding_grant_id":["5U01NS094296","P30 EY019007","W911NF-12-1-0594","CBET-0954796","U19 NS104649","R01HL13143801A1","U01 NS094296","R01 HL131438","UF1 NS108213"],"pubmed_authors":["Li W","Galwaduge PT","Voleti V","Benezra SE","Bruno RM","Perez-Campos C","Hillman EM","Yu H","Patel K"],"additional_accession":[]},"is_claimable":false,"name":"High-speed 3D imaging of cellular activity in the brain using axially-extended beams and light sheets.","description":"As optical reporters and modulators of cellular activity have become increasingly sophisticated, the amount that can be learned about the brain via high-speed cellular imaging has increased dramatically. However, despite fervent innovation, point-scanning microscopy is facing a fundamental limit in achievable 3D imaging speeds and fields of view. A range of alternative approaches are emerging, some of which are moving away from point-scanning to use axially-extended beams or sheets of light, for example swept confocally aligned planar excitation (SCAPE) microscopy. These methods are proving effective for high-speed volumetric imaging of the nervous system of small organisms such as Drosophila (fruit fly) and D. Rerio (Zebrafish), and are showing promise for imaging activity in the living m","dates":{"release":"2018-01-01T00:00:00Z","publication":"2018 Jun","modification":"2025-04-25T19:54:33.489Z","creation":"2019-07-01T13:40:34Z"},"accession":"S-EPMC6002850","cross_references":{"pubmed":["29642044"],"doi":["10.1016/j.conb.2018.03.007"]}}