biostudies-literatureHillman EMSimons Foundation Collaboration on the Global BrainNEI NIH HHSKavli Institute for Brain ScienceNHLBI NIH HHSCoulter FoundationColumbia-Coulter Translational Research PartnershipNINDS NIH HHSDepartment of Defense MURINational Institutes of HealthNational Science Foundation190-200https://www.ebi.ac.uk/biostudies/studies/S-EPMC6002850biostudies-literatureUnknown50As optical reporters and modulators of cellular activity have become increasingly sophisticated, the amount that can be learned about the brain via high-speed cellular imaging has increased dramatically. However, despite fervent innovation, point-scanning microscopy is facing a fundamental limit in achievable 3D imaging speeds and fields of view. A range of alternative approaches are emerging, some of which are moving away from point-scanning to use axially-extended beams or sheets of light, for example swept confocally aligned planar excitation (SCAPE) microscopy. These methods are proving effective for high-speed volumetric imaging of the nervous system of small organisms such as Drosophila (fruit fly) and D. Rerio (Zebrafish), and are showing promise for imaging activity in the living mCurrent opinion in neurobiologyHigh-speed 3D imaging of cellular activity in the brain using axially-extended beams and light sheets.PMC60028505U01NS094296P30 EY019007W911NF-12-1-0594CBET-0954796U19 NS104649R01HL13143801A1U01 NS094296R01 HL131438UF1 NS108213Li WGalwaduge PTVoleti VBenezra SEBruno RMPerez-Campos CHillman EMYu HPatel KfalseHigh-speed 3D imaging of cellular activity in the brain using axially-extended beams and light sheets.As optical reporters and modulators of cellular activity have become increasingly sophisticated, the amount that can be learned about the brain via high-speed cellular imaging has increased dramatically. However, despite fervent innovation, point-scanning microscopy is facing a fundamental limit in achievable 3D imaging speeds and fields of view. A range of alternative approaches are emerging, some of which are moving away from point-scanning to use axially-extended beams or sheets of light, for example swept confocally aligned planar excitation (SCAPE) microscopy. These methods are proving effective for high-speed volumetric imaging of the nervous system of small organisms such as Drosophila (fruit fly) and D. Rerio (Zebrafish), and are showing promise for imaging activity in the living m2018-01-01T00:00:00Z2018 Jun2025-04-25T19:54:33.489Z2019-07-01T13:40:34ZS-EPMC60028502964204410.1016/j.conb.2018.03.007