biostudies-literatureHouston CRosetrees TrustBritish Heart FoundationNational Institute for Health Research (NIHR)British Heart Foundation Centre of Research ExcellenceImperial CollegeAcademy of Medical Sciences155-164https://www.ebi.ac.uk/biostudies/studies/S-EPMC6004038biostudies-literatureUnknown119Fibrillation is the most common arrhythmia observed in clinical practice. Understanding of the mechanisms underlying its initiation and maintenance remains incomplete. Functional re-entries are potential drivers of the arrhythmia. Two main concepts are still debated, the "leading circle" and the "spiral wave or rotor" theories. The homogeneous subclone of the HL1 atrial-derived cardiomyocyte cell line, HL1-6, spontaneously exhibits re-entry on a microscopic scale due to its slow conduction velocity and the presence of triggers, making it possible to examine re-entry at the cellular level. We therefore investigated the re-entry cores in cell monolayers through the use of fluorescence optical mapping at high spatiotemporal resolution in order to obtain insights into the mechanisms of re-entrJournal of molecular and cellular cardiologyCharacterisation of re-entrant circuit (or rotational activity) in vitro using the HL1-6 myocyte cell line.PMC6004038RG/16/3/32175AMS-SGCL8-NgPG/15/59/31621M577PG/16/17/32069RE/13/4/30184CL-2011-21-001Tzortzis KNSaglietto APitcher DSChowdhury RADupont ERoney CCantwell CDNg FSPeters NSHouston CfalseCharacterisation of re-entrant circuit (or rotational activity) in vitro using the HL1-6 myocyte cell line.Fibrillation is the most common arrhythmia observed in clinical practice. Understanding of the mechanisms underlying its initiation and maintenance remains incomplete. Functional re-entries are potential drivers of the arrhythmia. Two main concepts are still debated, the "leading circle" and the "spiral wave or rotor" theories. The homogeneous subclone of the HL1 atrial-derived cardiomyocyte cell line, HL1-6, spontaneously exhibits re-entry on a microscopic scale due to its slow conduction velocity and the presence of triggers, making it possible to examine re-entry at the cellular level. We therefore investigated the re-entry cores in cell monolayers through the use of fluorescence optical mapping at high spatiotemporal resolution in order to obtain insights into the mechanisms of re-entr2018-01-01T00:00:00Z2018 Jun2025-04-07T00:05:21.591Z2019-03-26T23:42:29ZS-EPMC60040382974684910.1016/j.yjmcc.2018.05.002