biostudies-literatureUnknown293(24)Zuo XSpermatogenesis is precisely controlled by complex gene expression programs and involves epigenetic reprogramming, including histone modification and DNA methylation. SET domain-containing 2 (SETD2) is the predominant histone methyltransferase catalyzing the trimethylation of histone H3 lysine 36 (H3K36me3) and plays key roles in embryonic stem cell differentiation and somatic cell development. However, its role in male germ cell development remains elusive. Here, we demonstrate an essential role of <i>Setd2</i> for spermiogenesis, the final stage of spermatogenesis. Using RNA-seq, we found that, in postnatal mouse testes, <i>Setd2</i> mRNA levels dramatically increase in 14-day-old mice. Using a germ cell-specific <i>Setd2</i> knockout mouse model, we also found that targeted <i>Setd2</i>The Journal of biological chemistry9188-9197https://www.ebi.ac.uk/biostudies/studies/S-EPMC6005419biostudies-literatureThe histone methyltransferase SETD2 is required for expression of acrosin-binding protein 1 and protamines and essential for spermiogenesis in mice.PMC6005419Lan FRong BTong MHZuo XLv RLi LfalseThe histone methyltransferase SETD2 is required for expression of acrosin-binding protein 1 and protamines and essential for spermiogenesis in mice.Spermatogenesis is precisely controlled by complex gene expression programs and involves epigenetic reprogramming, including histone modification and DNA methylation. SET domain-containing 2 (SETD2) is the predominant histone methyltransferase catalyzing the trimethylation of histone H3 lysine 36 (H3K36me3) and plays key roles in embryonic stem cell differentiation and somatic cell development. However, its role in male germ cell development remains elusive. Here, we demonstrate an essential role of <i>Setd2</i> for spermiogenesis, the final stage of spermatogenesis. Using RNA-seq, we found that, in postnatal mouse testes, <i>Setd2</i> mRNA levels dramatically increase in 14-day-old mice. Using a germ cell-specific <i>Setd2</i> knockout mouse model, we also found that targeted <i>Setd2</i>2018-01-01T00:00:00Z2018 Jun2026-05-04T20:48:29.542Z2019-07-24T07:21:24ZS-EPMC60054192971699910.1074/jbc.RA118.00285110.1074/jbc.ra118.002851