biostudies-literatureCueto RNIDDK NIH HHSNHLBI NIH HHSNational Natural Science Foundation of ChinaNational Institutes of Health70-88https://www.ebi.ac.uk/biostudies/studies/S-EPMC6006524biostudies-literatureUnknown17Hyperhomocysteinemia (HHcy) is an independent risk factor for cardiovascular disease (CVD) which has been implicated in matochondrial (Mt) function impairment. In this study, we characterized Hcy metabolism in mouse tissues by using LC-ESI-MS/MS analysis, established tissue expression profiles for 84 nuclear-encoded Mt electron transport chain complex (nMt-ETC-Com) genes in 20 human and 19 mouse tissues by database mining, and modeled the effect of HHcy on Mt-ETC function. Hcy levels were high in mouse kidney/lung/spleen/liver (24-14 nmol/g tissue) but low in brain/heart (~5 nmol/g). S-adenosylhomocysteine (SAH) levels were high in the liver/kidney (59-33 nmol/g), moderate in lung/heart/brain (7-4 nmol/g) and low in spleen (1 nmol/g). S-adenosylmethionine (SAM) was comparable in all tissueRedox biologyIdentification of homocysteine-suppressive mitochondrial ETC complex genes and tissue expression profile - Novel hypothesis establishment.PMC6006524R01 HL116917HL9445R01 DK113775DK113775 (HW)HL108910HL82774R01 HL138749R01 HL077288DK104114HL77288R01 HL108910R01 HL13023381330004R01 HL132399HL116917 (XFY)F32 HL009445R01 HL131460HL67033R01 HL110764R01 HL117654HL131460R01 HL082774HL130233HL-110764R01 HL06703391639204 (YJ)Lavallee MShan HMZhang LWang HCueto RLi YFYang WYYang XLi XLopez JYu CHuang XJi YfalseIdentification of homocysteine-suppressive mitochondrial ETC complex genes and tissue expression profile - Novel hypothesis establishment.Hyperhomocysteinemia (HHcy) is an independent risk factor for cardiovascular disease (CVD) which has been implicated in matochondrial (Mt) function impairment. In this study, we characterized Hcy metabolism in mouse tissues by using LC-ESI-MS/MS analysis, established tissue expression profiles for 84 nuclear-encoded Mt electron transport chain complex (nMt-ETC-Com) genes in 20 human and 19 mouse tissues by database mining, and modeled the effect of HHcy on Mt-ETC function. Hcy levels were high in mouse kidney/lung/spleen/liver (24-14 nmol/g tissue) but low in brain/heart (~5 nmol/g). S-adenosylhomocysteine (SAH) levels were high in the liver/kidney (59-33 nmol/g), moderate in lung/heart/brain (7-4 nmol/g) and low in spleen (1 nmol/g). S-adenosylmethionine (SAM) was comparable in all tissue2018-01-01T00:00:00Z2018 Jul2026-04-29T05:05:27.642Z2019-03-26T23:42:32ZS-EPMC60065242967989310.1016/j.redox.2018.03.015