{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Du N"],"funding":["U.S. Department of Energy","Edna Bailey Sussman Foundation","Gordon and Betty Moore Foundation","Robert Buzzelli Endowed Fellowship"],"pagination":["e0199125"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC6008153"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["13(6)"],"pubmed_abstract":["The photosynthetic quantum yield (Φ), defined as carbon fixed or oxygen evolved per unit of light absorbed, is a fundamental but rarely determined biophysical parameter. A method to estimate Φ for both net carbon uptake and net oxygen evolution simultaneously can provide important insights into energy and mass fluxes. Here we present details for a novel system that allows quantification of carbon fluxes using pH oscillation and simultaneous oxygen fluxes by integration with a membrane inlet mass spectrometer. The pHOS system was validated using Phaeodactylum tricornutum cultured with continuous illumination of 110 μmole quanta m-2 s-1 at 25°C. Furthermore, simultaneous measurements of carbon and oxygen flux using the pHOS-MIMS and photon flux based on spectral absorption were carried out t"],"journal":["PloS one"],"pubmed_title":["Simultaneous quantum yield measurements of carbon uptake and oxygen evolution in microalgal cultures."],"pmcid":["PMC6008153"],"funding_grant_id":["GMBF3828","DE-EE0003373","DE-SC0008593"],"pubmed_authors":["Allen AE","Gholami P","Kline DI","DuPont CL","Mendola D","Mitchell BG","Martz T","Du N","Dickson AG"],"additional_accession":[]},"is_claimable":false,"name":"Simultaneous quantum yield measurements of carbon uptake and oxygen evolution in microalgal cultures.","description":"The photosynthetic quantum yield (Φ), defined as carbon fixed or oxygen evolved per unit of light absorbed, is a fundamental but rarely determined biophysical parameter. A method to estimate Φ for both net carbon uptake and net oxygen evolution simultaneously can provide important insights into energy and mass fluxes. Here we present details for a novel system that allows quantification of carbon fluxes using pH oscillation and simultaneous oxygen fluxes by integration with a membrane inlet mass spectrometer. The pHOS system was validated using Phaeodactylum tricornutum cultured with continuous illumination of 110 μmole quanta m-2 s-1 at 25°C. Furthermore, simultaneous measurements of carbon and oxygen flux using the pHOS-MIMS and photon flux based on spectral absorption were carried out t","dates":{"release":"2018-01-01T00:00:00Z","publication":"2018","modification":"2025-04-21T19:25:45.931Z","creation":"2019-03-26T23:42:34Z"},"accession":"S-EPMC6008153","cross_references":{"pubmed":["29920568"],"doi":["10.1371/journal.pone.0199125"]}}