{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Locke LW"],"funding":["NHLBI NIH HHS","NCI NIH HHS","Ohio State University College of Engineering","NIH"],"pagination":["201-210"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC6014630"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["108"],"pubmed_abstract":["Granulomas are the histopathologic hallmark of tuberculosis (TB), both in latency and active disease. Diagnostic and therapeutic strategies that specifically target granulomas have not been developed. Our objective is to develop a probe for imaging relevant immune cell populations infiltrating the granuloma. We report the binding specificity of Cyanine 3 (Cy3)-labeled cFLFLFK-PEG<sub>12</sub> to human leukocytes and cellular constituents within a human in vitro granuloma model. We also report use of the probe in in vivo studies using a mouse model of lung granulomatous inflammation. We found that the probe preferentially binds human neutrophils and macrophages in human granuloma structures. Inhibition studies showed that peptide binding to human neutrophils is mediated by the receptor form"],"journal":["Tuberculosis (Edinburgh, Scotland)"],"pubmed_title":["Use of a leukocyte-targeted peptide probe as a potential tracer for imaging the tuberculosis granuloma."],"pmcid":["PMC6014630"],"funding_grant_id":["P30 CA016058","T32 HL007946","P30 CA013148"],"pubmed_authors":["Chaney S","Tweedle M","Kothandaraman S","Schlesinger LS","Wozniak DJ","Locke LW"],"additional_accession":[]},"is_claimable":false,"name":"Use of a leukocyte-targeted peptide probe as a potential tracer for imaging the tuberculosis granuloma.","description":"Granulomas are the histopathologic hallmark of tuberculosis (TB), both in latency and active disease. Diagnostic and therapeutic strategies that specifically target granulomas have not been developed. Our objective is to develop a probe for imaging relevant immune cell populations infiltrating the granuloma. We report the binding specificity of Cyanine 3 (Cy3)-labeled cFLFLFK-PEG<sub>12</sub> to human leukocytes and cellular constituents within a human in vitro granuloma model. We also report use of the probe in in vivo studies using a mouse model of lung granulomatous inflammation. We found that the probe preferentially binds human neutrophils and macrophages in human granuloma structures. Inhibition studies showed that peptide binding to human neutrophils is mediated by the receptor form","dates":{"release":"2018-01-01T00:00:00Z","publication":"2018 Jan","modification":"2025-04-25T19:52:49.927Z","creation":"2019-03-26T22:31:24Z"},"accession":"S-EPMC6014630","cross_references":{"pubmed":["29623875"],"doi":["10.1016/j.tube.2018.01.001"]}}