biostudies-literatureLipsitz YYGouvernement du Canada | Natural Sciences and Engineering Research Council of CanadaCanada Research ChairsCIHR6369-6374https://www.ebi.ac.uk/biostudies/studies/S-EPMC6016797biostudies-literatureUnknown115(25)The development of cell-based therapies to replace missing or damaged tissues within the body or generate cells with a unique biological activity requires a reliable and accessible source of cells. Human pluripotent stem cells (hPSC) have emerged as a strong candidate cell source capable of extended propagation in vitro and differentiation to clinically relevant cell types. However, the application of hPSC in cell-based therapies requires overcoming yield limitations in large-scale hPSC manufacturing. We explored methods to convert hPSC to alternative states of pluripotency with advantageous bioprocessing properties, identifying a suspension-based small-molecule and cytokine combination that supports increased single-cell survival efficiency, faster growth rates, higher densities, and greaProceedings of the National Academy of Sciences of the United States of AmericaModulating cell state to enhance suspension expansion of human pluripotent stem cells.PMC6016797CGS-DTier 1Zandstra PWYin THanna JHLipsitz YYWoodford CfalseModulating cell state to enhance suspension expansion of human pluripotent stem cells.The development of cell-based therapies to replace missing or damaged tissues within the body or generate cells with a unique biological activity requires a reliable and accessible source of cells. Human pluripotent stem cells (hPSC) have emerged as a strong candidate cell source capable of extended propagation in vitro and differentiation to clinically relevant cell types. However, the application of hPSC in cell-based therapies requires overcoming yield limitations in large-scale hPSC manufacturing. We explored methods to convert hPSC to alternative states of pluripotency with advantageous bioprocessing properties, identifying a suspension-based small-molecule and cytokine combination that supports increased single-cell survival efficiency, faster growth rates, higher densities, and grea2018-01-01T00:00:00Z2018 Jun2026-05-06T06:47:31.249Z2019-03-26T23:43:48ZS-EPMC60167972986684810.1073/pnas.1714099115