biostudies-literatureUnknown23(3)Hus KK<i>Naja ashei</i> is an African spitting cobra species closely related to <i>N. mossambica</i> and <i>N. nigricollis</i>. It is known that the venom of <i>N. ashei</i>, like that of other African spitting cobras, mainly has cytotoxic effects, however data about its specific protein composition are not yet available. Thus, an attempt was made to determine the venom proteome of <i>N. ashei</i> with the use of 2-D electrophoresis and MALDI ToF/ToF (Matrix-Assisted Laser Desorption/Ionization Time of Flight) mass spectrometry techniques. Our investigation revealed that the main components of analysed venom are 3FTxs (Three-Finger Toxins) and PLA₂s (Phospholipases A₂). Additionally the presence of cysteine-rich venom proteins, 5'-nucleotidase and metalloproteinases has also been confirmed. The Molecules (Basel, Switzerland)E609https://www.ebi.ac.uk/biostudies/studies/S-EPMC6017371biostudies-literatureFirst Look at the Venom of Naja ashei.PMC6017371Petrilla VLyskowski ABocian AHus KKPetrillova MLegath JBuczkowicz JfalseFirst Look at the Venom of Naja ashei.<i>Naja ashei</i> is an African spitting cobra species closely related to <i>N. mossambica</i> and <i>N. nigricollis</i>. It is known that the venom of <i>N. ashei</i>, like that of other African spitting cobras, mainly has cytotoxic effects, however data about its specific protein composition are not yet available. Thus, an attempt was made to determine the venom proteome of <i>N. ashei</i> with the use of 2-D electrophoresis and MALDI ToF/ToF (Matrix-Assisted Laser Desorption/Ionization Time of Flight) mass spectrometry techniques. Our investigation revealed that the main components of analysed venom are 3FTxs (Three-Finger Toxins) and PLA₂s (Phospholipases A₂). Additionally the presence of cysteine-rich venom proteins, 5'-nucleotidase and metalloproteinases has also been confirmed. The 2018-01-01T00:00:00Z2018 Mar2026-04-08T02:32:42.727Z2019-03-27T00:06:37ZS-EPMC60173712951802610.3390/molecules23030609